人ProIGF-II E(78 - 88)抗血清通過用衍生自連接到白喉類毒素的人IGF-II E的E結構域的11個氨基酸的肽免疫兔來制備。 該序列對應于IGF-II E77 - 88,因此,抗血清識別全長IGF-II E和兩種IGF-II E變體(IGF-II E1 - 88和IGF-II E1 - 104),這兩種變體來源于人igf-2基因的加工和隨后的蛋白水解切割。 它不識別成熟的IGF-II或IGF-I。 序列分析表明,它可能識別非人靈長類動物、狗、豚鼠、豬和許多其他物種中的IGF-II E蛋白。 Human ProIGF-II E (78-88) antiserum was prepared by immunising rabbits with an 11 amino acid peptide derived from the E domain of human IGF-II E linked to diphtheria toxoid. The sequence corresponds to IGF-II E77-88and as a consequence, the antiserum recognises full length IGF-II E and both IGF-II E variants (IGF-II E1-88 andIGF-II E1-104)derived from processing of the human igf-2 gene and following proteolytic cleavage. It does not recognise mature IGF-II or IGF-I. Sequence analysis suggests it is likely to recognise IGF-II E proteins in non-human primates, dog, guinea-pig, pig and a number of other species.
GroPep Bioreagents公司總部位于澳大利亞,成立于1990年,致力于向廣大科研院所、生物技術及制藥公司開發、生產和銷售細胞培養級的活性生長因子(主要集中在胰島素樣生長因子(IGF)領域)、抗體及相關蛋白試劑,現有產品超過100多個,同時在世界范圍內均設有分銷商。公司在分子生物領域、發酵學及重組蛋白和單抗制備方面具有豐富的經驗,在制造工藝方面,擁有大量專利技術,GroPep目前已通過AS/NZS ISO 9001:2016質量管理體系認證。