人ProIGF-IE(71 - 86)抗血清通過用衍生自與白喉類毒素連接的人ProIGF-IE的E結構域的16個氨基酸的肽免疫兔來制備。 肽序列位于成熟IGF-I的C末端切割位點的緊下游,因此,抗血清識別所有來源于人igf-1基因可變剪接的ProIGF-I E蛋白。 它不識別成熟的IGF-I或IGF-II。 由于igf-1基因的選擇性剪接,它可能在非人靈長類動物、兔和豚鼠中識別ProIGF-I E蛋白,但在其它物種中不識別。 Human ProIGF-I E (71-86) antiserum was prepared by immunising rabbits with a 16 amino acid peptide derived from the E domain of human ProIGF-I E linked to diphtheria toxoid. The peptide sequence is located immediately downstream of the cleavage site at the C terminus of mature IGF-I and as a consequence, the antiserum recognises all ProIGF-I E proteins derived from alternative splicing of the human igf-1 gene. It does not recognise mature IGF-I or IGF-II. It is likely to recognise ProIGF-I E proteins in non-human primates, rabbits and guinea-pigs but not other species due to alternative splicing of the igf-1 gene.
GroPep Bioreagents公司總部位于澳大利亞,成立于1990年,致力于向廣大科研院所、生物技術及制藥公司開發、生產和銷售細胞培養級的活性生長因子(主要集中在胰島素樣生長因子(IGF)領域)、抗體及相關蛋白試劑,現有產品超過100多個,同時在世界范圍內均設有分銷商。公司在分子生物領域、發酵學及重組蛋白和單抗制備方面具有豐富的經驗,在制造工藝方面,擁有大量專利技術,GroPep目前已通過AS/NZS ISO 9001:2016質量管理體系認證。