該IGFBP-2抗體通過用衍生自與白喉類毒素連接的人IGFBP-2的C末端結構域的15個氨基酸的肽免疫兔來制備。 肽序列位于C末端上游,是IGFBP-2所特有的。 所述抗體識別IGFBP-2但不識別IGFBP-1、3、4、5、6或相關蛋白。 盡管該序列對人IGFBP-2是特異性的,但該抗體確實識別來自幾個物種的IGFBP-2,盡管這應該通過實驗來確定。 通過蛋白A親和層析純化免疫球蛋白級分。 該抗體適用于免疫測定、免疫印跡和免疫組織化學。 This IGFBP-2 antibody was prepared by immunising rabbits with a 15 amino acid peptide derived from the C-terminal domain of human IGFBP-2 linked to diphtheria toxoid. The peptide sequence is located upstream of the C terminus and is unique to IGFBP-2. The antibody recognises IGFBP-2 but not IGFBP-1, 3, 4, 5, 6 or related proteins. Although the sequence is specific to human IGFBP-2, the antibody does recognise IGFBP-2 from several species, although this should be experimentally determined. The immunoglobulin fraction was purified by Protein A affinity chromatography. The antibody is suitable for use in immunoassay, immunoblotting and immunohistochemistry.
GroPep Bioreagents公司總部位于澳大利亞,成立于1990年,致力于向廣大科研院所、生物技術及制藥公司開發、生產和銷售細胞培養級的活性生長因子(主要集中在胰島素樣生長因子(IGF)領域)、抗體及相關蛋白試劑,現有產品超過100多個,同時在世界范圍內均設有分銷商。公司在分子生物領域、發酵學及重組蛋白和單抗制備方面具有豐富的經驗,在制造工藝方面,擁有大量專利技術,GroPep目前已通過AS/NZS ISO 9001:2016質量管理體系認證。