該IGFBP-1抗體是通過用衍生自與白喉類毒素連接的人IGFBP-1的中心結構域的12個氨基酸的肽免疫兔而制備的。 該肽序列位于甲狀腺球蛋白1型結構域的緊鄰上游,是IGFBP-1所特有的。 該抗體識別人IGFBP-1,但不識別IGFBP-2、3、4、5或6或相關蛋白。 盡管該序列對人IGFBP-1是特異性的,但該抗體確實識別大鼠和小鼠IGFBP-1,并且也可在許多其它物種中起作用,盡管這應通過實驗確定。 通過蛋白A層析純化免疫球蛋白級分。 該抗體適用于免疫測定、免疫印跡和免疫組織化學。 This IGFBP-1 antibody was prepared by immunising rabbits with a 12 amino acid peptide derived from the central domain of human IGFBP-1 linked to diphtheria toxoid. The peptide sequence is located immediately upstream of the thyroglobulin type-1 domain and is unique to IGFBP-1. The antibody recognises human IGFBP-1 but not IGFBP-2, 3, 4, 5?or 6 or related proteins. Although the sequence is specific to human IGFBP-1, the antibody does recognise rat and mouse IGFBP-1 and may also work in a number of other species, although this should be experimentally determined. The immunoglobulin fraction was purified by Protein A chromatography. The antibody is suitable for use in immunoassay, immunoblotting and immunohistochemistry.
GroPep Bioreagents公司總部位于澳大利亞,成立于1990年,致力于向廣大科研院所、生物技術及制藥公司開發、生產和銷售細胞培養級的活性生長因子(主要集中在胰島素樣生長因子(IGF)領域)、抗體及相關蛋白試劑,現有產品超過100多個,同時在世界范圍內均設有分銷商。公司在分子生物領域、發酵學及重組蛋白和單抗制備方面具有豐富的經驗,在制造工藝方面,擁有大量專利技術,GroPep目前已通過AS/NZS ISO 9001:2016質量管理體系認證。