該IGFBP-5抗體是通過用衍生自人IGFBP-5的中心結構域的15個氨基酸的肽免疫兔而制備的,該中心結構域緊鄰1型甲狀腺球蛋白結構域的上游,并與白喉類毒素連接。 該肽序列是IGFBP-5特有的。 所述抗體識別IGFBP-5但不識別IGFBP-1、2、3、4、6或相關蛋白。 盡管該序列來源于人IGFBP-5,但該抗體確實識別來自幾個物種的IGFBP-5,盡管這應該通過實驗來確定。 通過蛋白A親和層析純化免疫球蛋白級分。 該抗體適用于免疫測定、免疫印跡和免疫組織化學。 This IGFBP-5 antibody was prepared by immunising rabbits with a 15 amino acid peptide derived from the central domain of human IGFBP-5, immediately upstream from the thyroglobulin-type 1 domain, linked to diphtheria toxoid. The peptide sequence is unique to IGFBP-5. The antibody recognises IGFBP-5 but not IGFBP-1, 2, 3, 4, 6 or related proteins. Although the sequence is derived from human IGFBP-5, the antibody does recognise IGFBP-5 from several species, although this should be experimentally determined. The immunoglobulin fraction was purified by Protein A affinity chromatography. The antibody is suitable for use in immunoassay, immunoblotting and immunohistochemistry.
GroPep Bioreagents公司總部位于澳大利亞,成立于1990年,致力于向廣大科研院所、生物技術及制藥公司開發、生產和銷售細胞培養級的活性生長因子(主要集中在胰島素樣生長因子(IGF)領域)、抗體及相關蛋白試劑,現有產品超過100多個,同時在世界范圍內均設有分銷商。公司在分子生物領域、發酵學及重組蛋白和單抗制備方面具有豐富的經驗,在制造工藝方面,擁有大量專利技術,GroPep目前已通過AS/NZS ISO 9001:2016質量管理體系認證。