FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)-FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

PRODUCT CENTER

細胞分析試劑盒

產品中心

當前位置：首頁 > 產品中心 > 細胞生物學產品 > 細胞分析試劑盒

中文名稱: FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)

英文名字: FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)

供應商: USBiological

產品貨號: F9103-52

產品報價: ￥1/100Tests

產品說明書: 點擊查看

購買方式: 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868

產品新聞

背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！

產品描述: FTO, Fat mass-and obesity-associated gene, was discovered as a responsible gene causing the mouse 'fused toes' mutation. The predicted 502aa Fto protein has a calculated molecular mass of 58kD and contains an N-terminal bipartite nuclear localization signal. RT-PCR detected Fto expression throughout mouse embryonic development and in all adult mouse tissues examined except heart and skin. Bioinformatics analysis indicates that FTO shares sequence motifs with iron- and 2-oxoglutarate (2OG)-dependent oxygenases. Recombinant murine Fto catalyzes the iron- and 2OG-dependent demethylation of 3-methylthymine in single-stranded DNA, with concomitant production of succinate, formaldehyde, and carbon dioxide. In wildtype mice, Fto mRNA is most abundant in the brain, particularly in hypothalamic nuclei, and are regulated by feeding and fasting. This assay is a sandwich ELISA for quantitative determination of mouse FTO in cells. A monoclonal antibody specific for FTO has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, FTO is recognized by the addition of a purified polyclonal antibody specific for FTO (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of FTO in the samples. This ELISA is specific for the measurement of natural and recombinant mouse FTO. It does cross-react with mouse adiponectin, mouse RBP4, mouse Nampt, mouse vaspin, mouse progranulin, mouse resistin, mouse clusterin, mouse GPX3, mouse CD137, rat adiponectin, rat Nampt, human adiponectin, human Nampt, human progranulin. The assay range is 0.156-10ng FTO/ml and a detection limit of 20pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).Sample Type:Cell lysates.Intended Use:For the quantitative determination of mouse FTO in cells.Sensitivity:20pg/ml Range:0.156-10ng FTO/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of mouse FTO in cells. A monoclonal antibody specific for FTO has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, FTO is recognized by the addition of a purified polyclonal antibody specific for FTO (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of FTO in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlLysis Buffer (10X): 1x12mlDetection Antibody: 1x12mlDetector 100X (HRP conjugated anti-IgG): 1x150ulMouse FTO Standard (lyophilized): 20ngMouse FTO QC Sample (lyophilized): 1 vialSubstrate Solution (TMB): 1x12mlStop Solution: 1x12mlPlate Sealers: 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產品特點: 針對FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)該產品的特點優勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)產品后將其置于4°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。