NEDD8 is part of the ubiquitin family. Human NEDD8 shares 60% amino acid sequence homology to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. NEDD8 is involved in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E-1 complex UbE1c- appbp1 and linkage to the E-2 enzyme UbE2m. Attachment of NEDD8 to cullins activates their associated E-3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
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來源宿主
大腸桿菌中重組表達
溶解建議
提供的人神經前體細胞表達發育下調基因-8(NEDD8)產品為過濾滅菌的無色溶液;其中人神經前體細胞表達發育下調基因-8(NEDD8)產品溶解于20mM Tris pH 8.0, 50mM NaCl, 0.5mM DTT,10%甘油溶液中.