Phospholipase A2（PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid（AA)， a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids， which represent another class of lipid mediators.
The secretory PLA2（sPLA2) family， in which 10 isozymes have been identified， consists of low molecular weight， Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes， such as modification of eicosanoid generation， inflammation， and host defense.
This enzyme has been proposed to hydrolyze phosphatidylcholine（PC) in lipoproteins to liberate lyso- PC and free fatty acids in the arterial wall， thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.
In mice， sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large: small ratio of surfactant aggregates in rats.
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來源宿主
大腸桿菌中重組表達
溶解建議
提供的人分泌磷脂酶A2-IID（PLA2G2D）產品為過濾滅菌的凍干粉末；該凍干粉末是從含0.5mg/ml人分泌磷脂酶A2-IID（PLA2G2D）的0.05M醋酸鹽緩沖液（pH 4）中過濾滅菌并凍干產生的.我們建議加0.2 ml 0.1M 醋酸鹽緩沖液（pH 4）以配制工作儲存液，使粉末完全溶解.如要使pH值更大，我們建議通過相關緩沖液進一步稀釋到10ug/ml.過高的濃度會影響PLA2G2D的溶解性.
