Pfu DNA polymeraseenzyme is found in the hyperthermophilic archaeonPyrococcus furiosus， where it functions in vivoto replicate the organism’s DNA. In vitro， Pfu is used to swiftly amplify DNAin the Polymerase Chain Reaction， where the enzyme serves the central function of copying a new strand of DNA during each extension step. Pfu DNA polymerase has superior thermostability and ’proofreading’ properties compared to other thermostable polymerases. Unlike Taq DNA polymerase， Pfu DNA polymerase possesses 3’ to 5’ exonuclease proof reading activity， meaning that it works its way along the DNA from the 5’ endto the 3’ endand corrects nucleotidemisin corporation errors. Pfu DNA polymerase-generated PCRfragments will have fewer errors than Taq-generated PCR inserts. As a result， Pfu is more commonly used for molecular cloning of PCR fragments than the historically popular Taq. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis， but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity.
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大腸桿菌中重組表達
溶解建議
提供的Pfu DNA聚合酶（Pfu DNA Polymerase）產品為滅菌的溶液形式；其中Pfu DNA聚合酶（Pfu DNA Polymerase）產品溶解于50mM Tris-HC1， pH 8.2， 1mM DTT， 0.1mM EDTA， 0.05% CHAPS，50%甘油溶液中.
保存建議
Pfu DNA聚合酶（Pfu DNA Polymerase）溶液可以在10℃保存5天；如果想延長保存時間，建議分裝后置于-18℃凍存.請避免反復凍融.
