這種新型的硫化氫（H2S）7-疊氮基-4-甲基香豆素（AzMC）選擇性探針可用于監測胱硫醚B-合酶（CBS）催化的反應，該反應在人類硫代謝中起關鍵作用。失活的CBS的特征是血漿高半胱氨酸和H2S水平高，導致許多疾病狀態的臨床表現。AzMC用作熒光探針和同時的CBS活性可用于選擇用于激活或抑制該酶的化合物，并且適合于高通量篩選和潛在治療劑的開發。此外，AzMC已被用作人酚磺基轉移酶（SULT 1A-1或P-PST-1）的底物結合位點的光親和探針。M.K.索爾森T. Majtan J. P. Kraus A.M. Barrios“使用硫化氫選擇性探針鑒定胱硫醚合酶抑制劑。“Angewandte Chemie（Int. ed. Eng.）2013年52 4641-4144。DOI：10.1002/anie.2013008412。G. Chen等人，“人類苯酚磺基轉移酶（SULT 1A 1）底物結合位點的光親和標記探針：7-疊氮基-4-甲基香豆素。蛋白質科學 1999 8 2151-2157 doi：10.1110/ps.8.10.21513. Q-L Xie等.“An azidocoumarin-based fluorescent probe for imaging lysosomal hydrogen sulfide in living cells”Analytical Methods 2017 DOI：10.1039/C7 AY 00862 G批量折扣可用，請發送電子郵件至echelon@echelon-inc.com獲取信息。
產品描述
This novel and selective probe for hydrogen sulfide (H2S) 7-azido-4-methylcoumarin (AzMC) is useful for monitoring cystathionine B-synthase (CBS) catalyzed reactions which play a critical role in human sulfur metabolism. Deactivated CBS is characterized by high plasma levels of homocysteine and H2S resulting in clinical manifestations of numerous disease states. AzMC used as a fluorgenic probe and concurrent CBS activity can be used to select compounds for activation or inhibition of this enzyme and is suitable for high-throughput screening and the development of potential therapeutics. In addition AzMC has been used as a photoaffinitry probe for the substrate binding site of human phenol sulfotransferase (SULT1A-1 or P-PST-1).Featured in References1. M.K. Thorson T. Majtan J.P. Kraus A.M. Barrios "Identification of Cystathionine ?2-Synthase Inhibitors Using a Hydrogen Sulfide Selective Probe." Angewandte Chemie (Int. ed. Eng.) 2013 52 4641-4144. DOI: 10.1002/anie.2013008412. G. Chen et al. "Photoaffinity labeling probe for the substrate binding site of human phenol sulfotransferase (SULT1A1): 7-azido-4-methylcoumarin." Protein Sci. 1999 8 2151-2157 doi:10.1110/ps.8.10.21513. Q-L Xie et a. "An azidocoumarin-based fluorescent probe for imaging lysosomal hydrogen sulfide in living cells" Analytical Methods 2017 DOI: 10.1039/C7AY00862G Bulk discounts available please email echelon@echelon-inc.com for information.
