膠原蛋白雜交肽是3-Hisperson Inc.的產品。膠原蛋白雜交肽（CHP）是一種新型獨特的肽，可在體外和體內特異性結合未折疊的膠原蛋白鏈[1 2 3]。通過共享天然膠原蛋白的Gly-X-Y重復序列，CHP具有通過以類似于退火至互補DNA鏈的DNA片段的方式重新形成三螺旋結構而與變性膠原蛋白鏈雜交的強能力。CHP非常具體：由于缺乏結合位點，它對完整的膠原蛋白分子具有可忽略的親和力，并且由于其中性和親水性，它對非特異性結合是惰性的。CHP是一種強大的組織病理學工具，能夠直接檢測由多種疾病引起的炎癥和組織損傷以及發育和衰老期間的組織重塑[3]。CHP可在不使用合成熒光基質或遺傳修飾細胞的情況下，在3D膠原蛋白培養物中通過癌細胞的蛋白水解遷移而穩健地可視化細胞周基質周轉[4]。CHP可以在分子水平上測量和定位膠原組織的機械損傷[5]。它還能夠評估脫細胞細胞外基質中的膠原蛋白變性[6]。此外，CHP可用于在SDS-PAGE凝膠中特異性顯示膠原蛋白條帶，而無需蛋白質印跡[7]。B-CHP用生物素標記，用于親和素/鏈霉親和素介導的檢測。B-CHP提供了靈活的檢測選擇，如非綠色熒光和HRP-酶法，以避免背景和增強信號。（目錄號：BIO）用于抗生物素蛋白/鏈霉抗生物素蛋白介導的方法，包括HRP-酶法5-FAM（目錄號：FLU），用于綠色熒光檢測和Cy 3（Catalog #：RED）用于紅色熒光檢測。應用：免疫熒光免疫組織化學細胞成像SDS-PAGE（凝膠內蛋白質印跡）運輸條件在環境溫度下以粉末形式快速運輸。儲存-20 ° C作為粉末; 4 °C在水中重構后無需等分和冷凍。在4 ° C溶液中穩定2年。參考文獻1）"Targeting and mimicking collagens via triple helical peptide moleclies" Curr Opin Chem Biol 2013 17 968- 975. 2）“Targeting collagen strand by photo-triggered triple-helix hybridization”Proceedings of the National Academy of Sciences of the United States of America 2012 109 14767-14772.?DOI：10.1073/pnas.1209721109.3）“用膠原雜交肽進行組織重塑的原位成像。ACS Nano 2017 11 9825。DOI：10.1021/acsnano.7b031504）“通過肽雜交可視化膠原蛋白水解：從3D細胞培養到體內成像?！癇iomaterials 2018 DOI：10.1016/j.biomerials.2018.08.0395）“膠原雜交肽實現膠原中機械損傷的分子水平檢測和定位?！秶彝ㄓ崱?2017 8 14913 DOI：10.1038/ncomms14913.6）“使用膠原雜交肽對脫細胞組織中的膠原變性進行分子評估?！吧锊牧蠈W報。 2017. 53 268-278 DOI：10.1016/j.actbio.2017.01.0797）“Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides” Bioconjugate Chemistry 2013 24 9-16. DOI：10.1021/bc 3005842.酶譜-比酶譜DQ膠原SHG和TEM更信息可靠和方便-對膠原的高親和力和無與倫比的特異性，基本上沒有非特異性結合-適用于依賴于膠原的二級結構而不是任何定義的結合序列的來自所有物種的所有類型的膠原-適用于冷凍和石蠟-包埋切片，無需抗原回收-無抗體方法，對任何共染色抗體沒有物種限制小尺寸（2%的IgG分子量），易于組織滲透和整個標本染色，無需切片-在4 °C以下的溶液中穩定，無需等分儲存
產品描述
The Collagen Hybridizing Peptides are products of 3-Helix Inc.The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains both in vitro and in vivo [1 2 3]. By sharing the Gly-X-Y repeating sequence of natural collagen CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites and it is inert towards non-specific binding because of its neutral and hydrophilic nature.CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases as well as tissue remodeling during development and aging [3]. CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture without the use of synthetic fluorogenic matrices or genetically modified cells [4]. CHP can measure and localize mechanical injury to collagenous tissue at the molecular level [5]. It also enables assessment of collagen denaturation in decellularized extracellular matrix [6]. In addition CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot [7]. B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options such as non-green fluorescence and HRP-enzyme methods to avoid background and enhance signal.CHP come in three varieties: labeled with biotin (Catalog #: BIO) for avidin/streptavidin-mediated including HRP-enzyme methods 5-FAM (Catalog #: FLU) for green fluorescence detection and Cy3 (Catalog #: RED) for red fluorescence detection.Applications: immunofluorescence immunohistochemistry cell imaging SDS-PAGE (in-gel Western blot)Shipping ConditionsExpress shipping as powder at ambient temperature. Store at -20 ?°C upon arrival until use.Storage-20 ?°C as powder; 4 ?°C after reconstitution in water no need to aliquot and freeze. Stable for 2 years in solution at 4 ?°C.References1) a€?Targeting and mimicking collagens via triple helical peptide assembliesa€? Curr Opin Chem Biol 2013 17 968-975.2) a€?Targeting collagen strand by photo-triggered triple-helix hybridizationa€? Proceedings of the National Academy of Sciences of the United States of America 2012 109 14767-14772. DOI: 10.1073/pnas.1209721109.3) "In situ imaging of tissue remodeling with collagen hybridizing peptides." ACS Nano 2017 11 9825. DOI: 10.1021/acsnano.7b031504) "Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging." Biomaterials 2018 DOI:10.1016/j.biomaterials.2018.08.0395) "Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides." Nat. Commun. 2017 8 14913 DOI: 10.1038/ncomms14913.6) "Molecular assessment of collagen denaturation in decellularized tissues using the collagen hybridizing peptide." Acta Biomater. 2017. 53 268-278 DOI: 10.1016/j.actbio.2017.01.0797) a€?Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptidesa€? Bioconjugate Chemistry 2013 24 9-16. DOI: 10.1021/bc3005842.Features-More informative reliable and convenient than zymography DQ collagen SHG and TEM-High affinity and unparalleled specificity to collagen with essentially no nonspecific binding-Applicable to all types of collagen from all species relying on collagen's secondary structure instead of any defined sequence for binding-Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval-A non-antibody approach with no species restrictions against any co-staining antibodySmall size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning-Stable in solution under 4 ?°C eliminating the need to aliquot for storage
