Cytochrome c is a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the bc1 complex and transfers electrons to the complex IV. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor-1 (Apaf-1). Cytochrome c can also catalyze several redox reactions such as hydroxylation and aromatic oxidation, and shows peroxidase activity by oxidation of various electron donors such as 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2-keto-4-thiomethyl butyric acid and 4-aminoantipyrine. BioVision’s Cytochrome c ELISA kit is a sandwich ELISA assay for the quantitative measurement of human Cytochrome c in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human Cytochrome c captured from the samples.
產品特點
Easy, convenient and time-saving method to measure the level of Cytochrome c in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 78.12 - 5000 pg/ml
Sensitivity: < 46.87 pg/ml
Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
