- 中文名稱
DNA, ss Apoptosis BioAssay ELISA Kit
- 英文名字
- DNA, ss Apoptosis BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- D3878-25
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The ssDNA Apoptosis ELISA Kit is a convenient, sensitive method for early detection of apoptosis. Principle:This procedure is based on the selective denaturation of DNA in apoptotic cells by formamide, and detection of denatured DNA with monoclonal antibody to single-stranded DNA (ssDNA). Formamide is a gentle agent that denatures DNA in apoptotic cells, but not in necrotic cells or in the cells with DNA breaks in the absence of apoptosis1. The sensitivity of DNA in apoptotic cells to formamide is not related to DNA breaks, but rather reflects changes in chromatin associated with apoptosis, such as chromatin condensation and digestion of proteins stabilizing DNA. The assay includes attachment of cells to 96-well plates, treatment of attached cells with formamide, and staining of ssDNA in apoptotic cells with a mixture of primary antibody and peroxidaselabeled secondary antibody. The protocol based on the one-step detection of ssDNA with antibody mixture has higher sensitivity and lower number of steps than standard two-step immunostaining2. This mixture is included in the kit in a ready to use form. The assay can be performed in two formats: 1. Cells are grown, treated with apoptosis inducing agents, stained and analyzed in the same microtiter plate. This assay is suitable for high throughput screening when induction of apoptosis is used as the endpoint of drug activity. 2. Cell suspensions obtained from cultures or tissues are transferred into a microtiter plate for staining and analysis. For this assay, cells could be fixed with methanol and stored before transfer into plates. Alternatively, fixation could be performed in microtiter plates after transfer of non-fixed cells. Total assay time is 3–4 hours, which includes fixation and staining. Only one washing step is required. The ssDNA Apoptosis ELISA is for research use only. Not for use in diagnostic or therapeutic procedures.Assay Characteristics Sensitivity:The high sensitivity of the ssDNA Apoptosis ELISA is characterized by1. Detection of 500 apoptotic cells per well and a linear relation between the absorbance and the number of apoptotic cells in the range of 500–5000 cells. 2. The ability to produce intense apoptosis signal by drug concentration inducing 50% growth inhibition, indicating that the assay sensitivity is sufficient for drug screening2. Specificity for Apoptosis over Necrosis The assay is highly specific for apoptotic cells as demonstrated by the absence of mAb reactivity with necrotic cells or with cells containing DNA breaks in the absence of apoptosis. Specificity of the assay was demonstrated using necrosis induced by hyperthermia or detergent and DNA breaks induced by hydrogen peroxide. These cells were completely negative by formamide-mAb assay, but positive by TUNEL1,2. Specificity is a major advantage of this anti-ssDNA mAb assay in comparison with the widely used TUNEL procedure. Universal Detection The detection of apoptotic cells with formamide-mAb procedure is based on the staining of condensed chromatin in apoptotic nuclei. Since chromatin condensation in compact masses is the most specific and definite hallmark of apoptosis, this method provides universal detection of apoptosis. The apoptotic events that occur without DNA breaks or without activation of specific caspases will be detected with our mAb to ssDNA. Kit Components:D3878-25A.. Antibody Mixture, 1x10ml. Ready to use. Primary monoclonal to ssDNA and HRP-labeled Anti-mouse IgMD3878-25B. Formamide, 1x5ml. Ready to use.D3878-25C. Positive Control, 1x2ml vial, 0.3ug/ml. Single-stranded DNA.D3878-25D. Wash Buffer Concentrate (10X), 1x10mlD3878-25E. ABTS,1x12ml. Ready to use solution of 2,2'-AZINO-bis (3-ethylbenziazoline-6-sulfonic acid) in a proprietary buffer with enhancer. D3878-25F. Stop Solution, 1x12ml HCl solution. Once the kit is opened, Antibody Mixture and ssDNA Positive Control should be thawed on ice, aliquoted and stored at-20o C. Formamide, Wash Buffer, ABTS Solution and Stop Solution can be thawed and stored at 4°C.
- 產品特點
- 針對DNA, ss Apoptosis BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到DNA, ss Apoptosis BioAssay ELISA Kit產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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