Anti-Host Cell Protein (HCP) Antibody Production, Option B - CUST93C-Anti-Host Cell Protein (HCP) Antibody Production, Option B

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中文名稱: Anti-Host Cell Protein (HCP) Antibody Production, Option B - CUST93C

英文名字: Anti-Host Cell Protein (HCP) Antibody Production, Option B - CUST93C

供應商: Rockland

產品貨號: CUST93C

產品報價: ￥詢價/1Each

產品說明書: 點擊查看

購買方式: 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868

產品新聞

背景資料: Complete service includes immunizing two different host species for anti-HCP antibody production to control for host species variability. Client selects two host animals: rabbit, chicken or sheep. Rabbits are valued as host animals due to the volume of historical data. Chicken/avian host show a varied pattern of immune response to mammalian HCP preparations especially CHO cell derived. Sheep may show greater immune sensitivity and yield larger volumes of antisera. HCP preparation is immunized in the two selected hosts. Project includes: 10 RABBIT or CHICKEN hosts or 3 SHEEP hosts, 90 days daily maintenance EACH, 10 Pre-bleeds EACH (up to 5 mL each), 20 Test bleeds EACH (up to 5 mL each), 8 to 20 production bleeds EACH (4 production bleeds from the 5 best responding RABBITS or CHICKENS or 4 production bleeds from the 2 best responding SHEEP) of up to 25 mL each (65 mL for SHEEP) or up to 500 mL). Only host animals showing reactivity to a broad range of proteins by size are selected for production. Terminal bleeds (1 each from the best responding 5 RABBITS or CHICKENS or 2 SHEEP of 100 mL each or up to 500 mL), western blotting of all test bleeds, production bleeds and terminal bleeds and against one (1) HCP lysate provided by client. After selection, host animal production bleeds are pooled and then purified by a method equivalent to Protein A chromatography (7S fractionation or IgY purification from eggs) up to 1.0 L of qualified antiserum. Vialing and lyophilization of up to 100 glass vials. Final qualification of final product by western blotting against provided HCP lysates. Summary report (1 page) with digital copy of finished product western blot and SDS-PAGE. Package charge for shipping of finished product is included.

產品描述: Complete service includes separation of the host cell protein into high molecular weight (HMW) and low molecular weight (LMW) fractions by ultra filtration, 10 RABBIT host animals, 90 days RABBIT daily maintenance, 10 RABBIT Pre-bleeds (5 mL each), 10 RABBIT test bleeds (5 mL each), 20 RABBIT production bleeds (4 production bleeds from the 5 best responding animals of 25 mL each or up to 500 mL), 5 Rabbit terminal bleeds (1 each from the 5 best responding animals of 100 mL each or up to 500 mL), western blotting of all test bleeds, production bleeds and terminal bleeds and against one (1) HCP lysate provided by client, Protein A purification of up to 1.0 L of qualified antiserum, vialing and lyophilization of up to 100 glass vials. Final qualification of final product by western blotting against provided HCP lysate. Summary report (1 page) with digital copy of finished product western blot and SDS-PAGE. Package charge for shipping of finished product is included.

產品形式: 該Anti-Host Cell Protein (HCP) Antibody Production, Option B - CUST93C的詳細信息查看Rockland提供的產品說明書

保存建議: 該Anti-Host Cell Protein (HCP) Antibody Production, Option B - CUST93C的詳細信息查看Rockland提供的產品說明書

其他: Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。