Streptavidin is used extensively in molecular biology and bionanotechnology due to its extraordinarily high affinity for biotin (dissociation constant in the femtomolar range). The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is a 52.8 KDa homo-tetramer protein purified from the bacterium Streptomyces avidin. This personalized kit is designed for streptavidin to work with any IgG antibody. Using the kit components, customers convert their antibody (not included) to a thiol-antibody, which is then reacted with activated streptavidin (provided in kit) to generate the streptavidin-antibody conjugates. The combination of filtration and scavenger-type purification steps typically provides the resulting streptavidin-antibody at greater than 90% purity.
This kit provides materials to label one (CM52420x1) to three (CM52420x3) antibody samples.
Total amount of activated streptavidin included in a reaction: 2 mg. Amount of IgG needed: 1 mg per reaction.
