中文名稱

MAL-dPEG

英文名字

MAL-dPEG???-TFP ester-1000 mg

供應商

Click Chemistry Tools

產品貨號

CCT-QBD-10553-1000

產品報價

￥詢價/1000mg

產品說明書

點擊查看

購買方式

銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系。本公司所有產品僅供科研使用，不用于臨床診斷。

產品新聞

背景資料

MAL-dPEG?12-TFP酯(產品編號QBD-10553)是一種交聯劑，可通過中等長度(49個原子)、單分子量、離散PEG(dPEG?)接頭將游離胺連接至游離巰基(巰基)。巰基通過邁克爾加成反應與馬來酰亞胺基反應。胺通過末端丙酸基團的2，3，5，6-四氟苯基(TFP)酯的親核取代與交聯劑形成酰胺鍵。

產品描述

MAL-dPEG?12-TFP ester, product number QBD-10553, is a crosslinking reagent that joins free amines to free thiols (sulfhydryl groups) across a medium-length (49 atoms), single molecular weight, discrete PEG (dPEG?) linker. The sulfhydryl groups react with a maleimido group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the 2,3,5,6-tetrafluorophenyl (TFP) ester of the terminal propionic acid group.nThe conjugation of free amines to free thiols is one of the most popular, most useful crosslinking reactions in bioconjugate chemistry is the conjugation of free amines to free thiols. These reactions require heterobifunctional reagents that bridge the two groups. Typical crosslinkers are hydrophobic. Vector Laboratories' dPEG? crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths. These hydrophilic, non-immunogenic, dPEG? crosslinkers prevent the problems with aggregation that arise when using traditional, hydrophobic crosslinkers.nPublished studies have shown that TFP esters are more hydrolytically stable in aqueous buffers than N-hydroxysuccinimidyl (NHS) esters and are more reactive toward free amines. TFP esters react under the same conditions as NHS esters. However, the optimal pH range for TFP esters (7.5 – 8.0) is slightly higher than for NHS esters (7.0 – 7.5). Amide bond formation between the TFP-activated propionic acid group of MAL-dPEG?12-TFP ester and a free amine will be slightly slower at a suboptimal pH compared to the reaction rate within the optimum pH range.nThe maleimide end of MAL-dPEG?12-TFP ester, product number QBD-10553, reacts optimally with a sulfhydryl at pH 6.5 – 7.5. Conduct the conjugation at the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which may confound the data. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.nMAL-dPEG?12-TFP ester, product number QBD-10553, can be used the same way and in the same applications as the equivalent NHS esters. Possible uses for this product include the following:nCoating nanoparticle surfaces;nTethering antibodies to atomic force microscopy (AFM) probes;nConstructing antibody-drug conjugates (ADCs);nAdding flexibility and water solubility to a peptide;nIncreasing the water solubility and hydrodynamic volume of hydrophobic biomolecules;nBuilding supramolecular constructs; and,nConjugating the TFP ester end of the molecule a liposomal surface and then using the free maleimide end of the molecule to attach a small molecule drug, peptide, or antibody for targeted delivery of a diagnostic or therapeutic package.

產品形式

無色至琥珀色油狀物;在儲存條件下可能固化為蠟狀固體

保存建議

其他

Click Chemistry Tools（CCT）公司位于美國亞利桑那州斯科茨代爾（Scottsdale,AZ）。自公司成立以來，一致致力于不斷開發和擴展一系列連接產品。他們利用特別新的研究成果研發生產出新穎的交聯和標記試劑。產品被廣泛用于藥物研發、癌癥治療以及各類診斷技術等。此外，CCT還可以提供個性化的定制產品，已滿足不同用戶的需求。目前CCT的產品線主要包括：Azides（Biotin-Azides）試劑、Bis-sulfone交聯試劑、DBCO試劑、TCO試劑、Terminal Alkyne試劑、Tetrazine試劑、標記試劑盒以及分選用底物等。nFluoroprobes公司位于美國亞利桑那州斯科茨代爾(Scottsdale, AZ)，自成立以來，一直致力于不斷開發一系列的連接(linker)產品。利用特別新的研究成果研發生產出各類新穎的交聯和標記試劑。其產品被廣泛用于藥物研發與篩選、疾病治療方法和診斷技術等領域。此外，Fluoroprobes還可以根據客戶的個性化需求提供各類定制連接產品。代表產品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些標記試劑盒等。nQuanta Biodesign公司位于美國中部的俄亥俄州，成立于1997年。也是專注于生產研發各類標記產品的品牌之一。其研發的產品與dPEG技術相結合，生產出他們獨有的專利產品dPEG Building Blocks and Linkers，被廣泛用于細胞治療和分子診斷等研究領域。另外，還可為廣大科研工作者提供多種化學修飾試劑以及傳統Liner等。n目前上述三個品牌都已被被Vectorlabs收購并整合到Vectorlabs產品中

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

分享到：