TFP-dPEG? β-生物素酶抗性生物素-100 mg-TFP-dPEG??-biotinidase resistant biotin-100 mg代理/參數/廠家-生化試劑-艾美捷科技有限公司

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中文名稱: TFP-dPEG? β-生物素酶抗性生物素-100 mg

英文名字: TFP-dPEG??-biotinidase resistant biotin-100 mg

供應商: Click Chemistry Tools

產品貨號: CCT-QBD-10203-100

產品報價: ￥詢價/100mg

產品說明書: 點擊查看

購買方式: 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系。本公司所有產品僅供科研使用，不用于臨床診斷。

產品新聞

背景資料: TFP-dPEG?4-生物素酶抗性生物素，產品編號QBD-10203，是一種經修飾以抵抗生物素酶水解的水溶性生物素化試劑(EC 3.5.1.12)。該產品將生物素?；?2-氨基丁酸與單分子量離散聚乙二醇(dPEG?)連接體的2，3，5，6-四氟苯基(TFP)酯結合。TFP-dPEG?4-生物素酶抗性生物素保留了生物素對抗生物素蛋白和鏈霉抗生物素蛋白的典型高親和力，但也阻斷了生物素酶對生物素的作用。該產品可用于組織樣品的ELISA和抗體預靶向等應用，其中內源性生物素酶可回收生物素。

產品描述: TFP-dPEG?4-biotinidase-resistant biotin, product number QBD-10203, is a water-soluble biotinylation reagent modified to resist hydrolysis by biotinidase (EC 3.5.1.12). This product combines Biotinoyl-2-aminobutyric acid with the 2,3,5,6-tetrafluorophenyl (TFP) ester of a single molecular weight, discrete polyethylene glycol (dPEG?) linker. TFP-dPEG?4-biotinidase-resistant biotin retains biotin’s typical high affinity for avidin and streptavidin but also blocks the action of biotinidase on biotin. This product is useful in applications such as ELISAs of tissue samples and antibody pretargeting, where endogenous biotinidase can recycle biotin.nBiotinidase is an amidohydrolase that breaks the covalent bond between biotin and amino acids such as lysine (i.e., biocytin). In vivo, biotinidase recycles biotin. Although necessary for healthy bodily function, biotinidase potentially creates problems when biotinylated proteins are used in serological assays, since endogenous biotinidase removes the biotin from these proteins.nDr. D. Scott Wilbur and colleagues at the University of Washington in Seattle developed biotinidase-resistant biotin in 2006 to facilitate in vivo antibody pretargeting. However, to improve the product’s water solubility, a short amino-dPEG?4-propionic acid linker was conjugated to the biotinidase-resistant biotin and subsequently functionalized as an TFP ester. This product is essentially the same as QBD-10202, but the TFP ester is more stable to hydrolysis in aqueous media and has a higher optimal pH range of 7.5 - 8.5 compared to NHS esters.nLike our other dPEG? biotinylation products, TFP-dPEG?4-biotinidase-resistant biotin, QBD-10203, will not cause aggregation and precipitation of biomolecules to which it is conjugated, and the dPEG? spacer reduces non-specific binding.nTFP-dPEG?4-biotinidase-resistant biotin resists amide hydrolysis because of the non-natural amino acid to which it is conjugated. It is potentially beneficial for applications using serological fluids that contain endogenous biotinidase. Useful roles for TFP-dPEG?4-biotinidase-resistant biotin include the following:ntherapeutic applications such as antibody pretargeting with biotin-streptavidin systems;nsupramolecular constructs (e.g., nanoparticles) used in vivo that depend on biotin-(strept)avidin affinity to maintain structural integrity; and,nELISAs of serological fluids and tissue samples using biotin-(strept)avidin affinity.

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保存建議

其他: Click Chemistry Tools（CCT）公司位于美國亞利桑那州斯科茨代爾（Scottsdale,AZ）。自公司成立以來，一致致力于不斷開發和擴展一系列連接產品。他們利用特別新的研究成果研發生產出新穎的交聯和標記試劑。產品被廣泛用于藥物研發、癌癥治療以及各類診斷技術等。此外，CCT還可以提供個性化的定制產品，已滿足不同用戶的需求。目前CCT的產品線主要包括：Azides（Biotin-Azides）試劑、Bis-sulfone交聯試劑、DBCO試劑、TCO試劑、Terminal Alkyne試劑、Tetrazine試劑、標記試劑盒以及分選用底物等。nFluoroprobes公司位于美國亞利桑那州斯科茨代爾(Scottsdale, AZ)，自成立以來，一直致力于不斷開發一系列的連接(linker)產品。利用特別新的研究成果研發生產出各類新穎的交聯和標記試劑。其產品被廣泛用于藥物研發與篩選、疾病治療方法和診斷技術等領域。此外，Fluoroprobes還可以根據客戶的個性化需求提供各類定制連接產品。代表產品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些標記試劑盒等。nQuanta Biodesign公司位于美國中部的俄亥俄州，成立于1997年。也是專注于生產研發各類標記產品的品牌之一。其研發的產品與dPEG技術相結合，生產出他們獨有的專利產品dPEG Building Blocks and Linkers，被廣泛用于細胞治療和分子診斷等研究領域。另外，還可為廣大科研工作者提供多種化學修飾試劑以及傳統Liner等。n目前上述三個品牌都已被被Vectorlabs收購并整合到Vectorlabs產品中