中文名稱

AZDye 800Azide吡啶-5 mg

英文名字

AZDye 800 Picolyl Azide-5 mg

供應商

Click Chemistry Tools

產品貨號

CCT-1563-5

產品報價

￥詢價/5mg

產品說明書

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購買方式

銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系。本公司所有產品僅供科研使用，不用于臨床診斷。

產品新聞

背景資料

AZDye? 800 Picolyl Azide是一種先進的熒光探針，它結合了銅螯合基序，以提高反應位點處Cu(I)的有效濃度，從而提高CuAAC反應的效率，從而實現更快、更生物相容的CuAAC標記。據報道，與常規疊氮化物相比，信號強度增加高達40倍。

產品描述

Cyanine heptamethine dyes are well-known NIR fluorophores, with emission wavelengths >740 nm, and often are reagents of choice for labeling antibodies. Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800. While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability.3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity.4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent.8,9 Moreover, the close stacking of proximal conjugated IRDye 800CW or DyLight800 on an antibody surface amplifies the potential for a deleterious effect on antibody targeting because of a localized patch of polyanionic charge and hydrophobicity.nnIn order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory.10, 11 These dyes contain two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus enhance the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface.10, 11nnThese sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.nnMB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces.12,13

產品形式

綠色固體

保存建議

-20

其他

Click Chemistry Tools（CCT）公司位于美國亞利桑那州斯科茨代爾（Scottsdale,AZ）。自公司成立以來，一致致力于不斷開發和擴展一系列連接產品。他們利用特別新的研究成果研發生產出新穎的交聯和標記試劑。產品被廣泛用于藥物研發、癌癥治療以及各類診斷技術等。此外，CCT還可以提供個性化的定制產品，已滿足不同用戶的需求。目前CCT的產品線主要包括：Azides（Biotin-Azides）試劑、Bis-sulfone交聯試劑、DBCO試劑、TCO試劑、Terminal Alkyne試劑、Tetrazine試劑、標記試劑盒以及分選用底物等。nFluoroprobes公司位于美國亞利桑那州斯科茨代爾(Scottsdale, AZ)，自成立以來，一直致力于不斷開發一系列的連接(linker)產品。利用特別新的研究成果研發生產出各類新穎的交聯和標記試劑。其產品被廣泛用于藥物研發與篩選、疾病治療方法和診斷技術等領域。此外，Fluoroprobes還可以根據客戶的個性化需求提供各類定制連接產品。代表產品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些標記試劑盒等。nQuanta Biodesign公司位于美國中部的俄亥俄州，成立于1997年。也是專注于生產研發各類標記產品的品牌之一。其研發的產品與dPEG技術相結合，生產出他們獨有的專利產品dPEG Building Blocks and Linkers，被廣泛用于細胞治療和分子診斷等研究領域。另外，還可為廣大科研工作者提供多種化學修飾試劑以及傳統Liner等。n目前上述三個品牌都已被被Vectorlabs收購并整合到Vectorlabs產品中

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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