- 中文名稱
Cytotoxicity Assay Kit, BioAssay
- 英文名字
- Cytotoxicity Assay Kit, BioAssay
- 供應商
- USBiological
- 產品貨號
- C9130-02
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Adenosine 5'-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells.ATP+D-luciferin+O2 ----luciferase--->oxyluciferin+AMP+PPi+CO2+lightThis non-radioactive, homogeneous cell-based assay can be conveniently performed in microplates. The reagent is compatible with all culture media and liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.Key Features:Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).Sensitive and accurate. As low as 50 cells can be quantified.Homogeneous and convenient. Mix-incubate-measure type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.Applications:Cell proliferation: effects of cytokines, growth factor, nutrients.Cytotoxicity and apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc.Drug discovery: high-throughput screening for anticancer drugs.Kit Contents:Assay Buffer, 1x10mlSubstrate, 1x120ulATP Enzyme, 1x120ulStorage and Stability:Store ?components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.? Assay Procedure: In 96-Well Plates1. Cell Culture. Plate cells at 100ul/well in white opaque tissue culture plates. If desired, add 5ul test compounds and controls dissolved in PBS or culture medium per well. Rock plate lightly to mix and incubate for desired period of time (e.g. overnight).2. Assay. Bring all components to room temperature. Keep thawed ATP Enzyme on ice or 4°C. For each test well, mix 95ul Assay Buffer with 1ul Substrate and 1ul ATP Enzyme. Add 90ul Reconstituted Reagent to each test well and mix by tapping the plate. Incubate for 2 minutes at room temperature. Read luminescence on a luminometer. For most luminometers (Berthold Luminometer, LJL Analyst, Top Count, MicroBeta Counters, CLIPR and LeadSeeker), integration time of 0.1 to 5 sec is appropriate.Assay Procedure: In 384-Well Plates1. Cell Culture. Plate cells at 25ul/well in white opaque tissue culture plates. If desired, add 5ul test compounds and controls dissolved in PBS or culture medium per well. Rock plate lightly to mix and incubate for desired period of time (e.g. overnight).2. Assay. Bring all components to room temperature. Keep thawed ATP enzyme on ice or 4°C. For each test well, mix 30ul Assay Buffer with 0.3ul Substrate and 0.3ul ATP Enzyme. Add 25ul Reconstituted Reagent to each well and mix by tapping the plate. Incubate for 2 minutes at room temperature.Read luminescence on a luminometer. For most luminometers (Berthold Luminometer, LJL Analyst, Top Count, MicroBeta Counters, CLIPR and LeadSeeker), integration time of 0.1 to 5 sec is appropriate.General Considerations:Signal stability. After adding the Reconstituted Reagent, the luminescence signal is stable for about 15 min and decreases slow thereafter. Reading is best performed within 30 min.
- 產品特點
- 針對Cytotoxicity Assay Kit, BioAssay該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Cytotoxicity Assay Kit, BioAssay產品后將其置于-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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