- 中文名稱
Cell Viability Assay Kit, BioAssay
- 英文名字
- Cell Viability Assay Kit, BioAssay
- 供應商
- USBiological
- 產品貨號
- C2609-24
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
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- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Therefore, assays for calculating cell viability are necessary for optimizing cell culture conditions, evaluating cell growth factors and nutrients, discovering novel antibiotics and anti-cancer drugs, evaluating toxic effects of environmental pollutants and cell mediated toxicity and studying programmed cell death (apoptosis).The Cell Viability assay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous Colorimetric Assay: is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. This cellular reduction reaction involves the pyridine nucleotide cofactors NADH/NADPH and is only catalyzed by living cells. The formazan product has a low aqueous solubility and is present as purple crystals. Dissolving the resulting formazan with a solubilization buffer permits the convenient quantification of product formation. The intensity of the product color, measured at 550-620nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation.Key Features:Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).Sensitive and accurate. As low as 950 cells can be accurately quantified.Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day.Homogeneous and convenient. Mix-incubate-measure type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS. Z’ factors of 0.5 and above are observed.Can be readily automated with HTS liquid handling systems.Applications:Cell Proliferation: effects of cytokines, growth factor, nutrients.Cytotoxicity and Apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc.Drug Discovery: high-throughput screen for toxic and anticancer drugs.Kit Contents:Assay BufferSolubilization Solution Note: Control Reagent: Saponin not supplied with kitStorage Conditions:Store the Reagent at -20°C. Keep Assay Buffer and Solubilization Solution at 4 °C and room temperature, respectively. Shelf life: 12 month after receipt.Precautions:Reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:The Cell Viability assay is based on the conversion of MTT to purple formazan by metabolically active cells. For most cells, this reducing reaction takes 3 to 5 hours. The produced formazan is solubilized and the resulting colored solution is quantified with a microplate reader. Although most culture media contain phenol red, phenol red does not interfere with the assay. All data in Technical Notes: were obtained in culture media containing phenol red.Procedure using 96-well plate:1. Plate and culture cells (80ul per well) in a clear bottom 96-well tissue culture plates. Typical culture medium contains DMEM, 10% fetal bovine serum, antibiotics (penicillin/streptomycin, gentamycin, etc), amino acids and other nutrients. Assays can be performed on either adherent cells or cells in suspension. The number of cells can vary from 1000 to 80,000 per well. The volume can vary from 50 to 150ul, although 80ul is used in this example. In addition to the test samples, one must include control wells of culture medium containing no cells or cells treated with a toxic reagent such as 0.1% saponin.2. Add test compounds and controls and incubate cells for the desired period of time (typically overnight). It is recommended that assays be run in duplicate or triplicate. A volume of 20ul in phosphate buffered saline (PBS) or culture medium is recommended for the test compounds and controls. The Control reagent can be conveniently reconstituted with 5ml PBS (1% saponin).3. Reconstitute the Reagent with Assay Buffer. First equilibrate the Reagent and Assay Buffer to room temperature. Then simply combine Assay Buffer and the Reagent by pipetting a small volume (e.g. 1ml) buffer to the Reagent tube. Vortex briefly and pipet the reconstituted solution to the Assay Buffer bottle. Repeat this step to transfer all Reagent to the Assay Buffer bottle. Mix by inversion until the Reagent is thoroughly dissolved. After this is done, mark the bottle label as Reconstituted Reagent. Note: Fresh reconstitution is recommended although the reconstituted reagent is stable for up to 4 weeks when stored at -20 °C.4. Add 15ul (per 80ul cell culture) of reagent per well and incubate for 4 hours at 37°C. The volume of the reagent should be adjusted depending on the volume of cell culture.5. Add 100ul of the Solubilization Solution. Mix gently on an orbital shaker for one hour at room temperature. The volume of the Solubilization Solution should be adjusted depending on the volume of cell culture. If precipitation occurs in the Solubilization Solution, place the bottle in a warm water bath or at 37°C and shake to dissolve precipitates.6. Measure OD570nm for each well on an absorbance plate reader. Maximum absorbance of the formazan dye lies between 560 and 590nm. If desired, the OD measurement can be performed the following day. In this case, it is recommended to seal the plate to minimize evaporation.Data Analysis:Determine the average of the blank controls and subtract this amount from all absorbance values. Plot the corrected absorbance values at 570nm against the concentration of the test compound. Determine the EC50 value for cell proliferation and IC50 value for cytotoxic compound by non-linear regression analysis using Prism or any other Data Analysis: tools.Technical Notes:The Cell Viability assay kit provides a convenient and homogeneous assay for cell proliferation and cytotoxicity in multi-well plates. Reagents of the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation. Key Features: of the kits include:Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).Sensitive and accurate. As low as 950 cells can be accurately quantified.Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day.Homogeneous and convenient. Mix-incubate-measure type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of 0.5 and above are observed.Can be readily automated with HTS liquid handling systems.
- 產品特點
- 針對Cell Viability Assay Kit, BioAssay該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Cell Viability Assay Kit, BioAssay產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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