CEA, BioAssay ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembryonic Antigen, Meconium Antigen 100, CD66e, CEACAM5)-CEA, BioAssay ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembryonic Antigen, Meconium Antigen 100, CD66e, CEACAM5)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: CEA, BioAssay ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembryonic Antigen, Meconium Antigen 100, CD66e, CEACAM5)

英文名字: CEA, BioAssay ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembryonic Antigen, Meconium Antigen 100, CD66e, CEACAM5)

供應商: USBiological

產品貨號: C1300-06A

產品報價: ￥1/96Tests

產品說明書: 點擊查看

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產品描述: Carcinoembryonic antigen (CEA) was first described in 1965 by Gold and Freedman when they identified an antigen that was present in both fetal colon and colon adenocarcinoma but that appeared to be absent from healthy adult colon. CEA is a glycoprotein containing approximately 50% carbohydrate with a molecular weight of 180kD. CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed.CEA has proven to be a suitable target antigen for the detection of primary and metastatic colorectal and some other carcinomas. Although sera from healthy individuals and from patients with other diseases generally had low levels of CEA, CEA is one of the most widely used tumor markers worldwide. Its main application is mostly in gastrointestinal cancers, especially in colorectal malignancy. CEA assays are now generally accepted clinically as a useful and cost- efficient tool in monitoring of colon cancer following surgery.Intended Use:Human Carcinoembryonic Antigen (human CEA) ELISA kit is to be used for the in vitro quantitative determination of human CEA in human serum, human plasma, cell lysate and buffered solution. The assay will recognize native CEA. This kit has been configured for research use only and is not to be used in diagnostic procedures.Sensitivity:The minimal detectable dose of human CEA was calculated to be 0.15ng/ml, by subtracting two standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.Specificity:The following substances were tested and found to have no cross-reactivity: humanTest Principle:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human CEA. Samples are pippetted into these wells. Nonbound CEA and other components of the sample should be removed by washing, then biotin-conjugated monoclonal antibody specific to CEA added. In order to quantitatively determine the amount of CEA present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured CEA.Kit Components:Microplate: 1x96 wellsIncubation buffer: 1x30mlWashing buffer, (10x): 1x100mlStandard protein: 1 glass vial lyophilizedStandard/sample dilution buffer: 1x25mlSecond antibody: 1 glass vial lyophilizedAV-HRP: 1x150ulSecondary antibody/AV-HRP dilution buffer: 1x25mlSubstrate (TMB): 1x20mlStop solution: 1x20mlStorage and Stability:Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產品特點: 針對CEA, BioAssay ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembryonic Antigen, Meconium Antigen 100, CD66e, CEACAM5)該產品的特點優勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到CEA, BioAssay ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembryonic Antigen, Meconium Antigen 100, CD66e, CEACAM5)產品后將其置于4°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。