- 中文名稱
TrueBlack?脂褐素自體熒光淬滅劑，20 X DMF溶液
- 英文名字
- TrueBlack? Lipofuscin Autofluorescence Quencher, 20X in DMF
- 供應商
- Biotium
- 產品貨號
- BTM-23007
- 產品報價
- ￥詢價/1ML
- 產品說明書
- 點擊查看
- 購買方式
- 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- Features:r
r - Eliminates lipofuscin autofluorescence
r - Reduces autofluorescence from non-lipofuscin sources
r - Doesn't cause high background, unlike Sudan Black B
r - Can be used before or after immunofluorescence staining
r - Clears the way for fluorescence imaging of human and aged animal tissues
r
r TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF, is a new reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy, and accumulate in a variety of cell and tissue types with age. Consequently, imaging of specific immunofluorescence signal in certain adult human tissues or aged animal tissues can be virtually impossible unless methods are employed to quench or mask lipofuscin fluorescence.r r Traditionally, Sudan Black B has been used to quench lipofuscin autofluorescence by incubating tissue sections with the dye after immunofluorescence staining. However, while it masks the autofluorescence from lipofuscin, Sudan Black B also introduces uniform non-specific background fluorescence in the red and far-red channels, limiting the use of fluorescent dyes in those wavelengths. Now Biotium has developed TrueBlack® as a superior alternative to Sudan Black B. TrueBlack® quenches lipofuscin fluorescence with far less increase in red/far-red background fluorescence. TrueBlack® treatment can be performed before or after immunostaining. It is rapid, simple, and has minimal effect on signal from fluorescent antibodies or nuclear counterstains, thus preserving the signal-to-noise ratio of the immunostaining.r r TrueBlack® effectively eliminates lipofuscin autofluorescence in tissues like human and mouse brain and retina.? TrueBlack® also can reduce autofluorescence from other sources, such as collagen, elastin, red blood cells, and general background fluorescence. It is not as effective at quenching these sources of autofluorescence as it is for lipofuscin, but it can improve background in human and non-human tissue types. TrueBlack® also has been used to quench background fluorescence on polycarbonate filters used as cell supports for imaging cytometry.
- 產品描述
- TrueBlack®可有效消除人和小鼠大腦和視網膜等組織中的脂褐素自發熒光。 TrueBlack®還可以減少來自其他來源的自發熒光，例如膠原蛋白，彈性蛋白，紅細胞和一般背景熒光。它在淬滅這些自發熒光源方面不如它對脂褐素有效，但它可以改善人類和非人類組織類型中的背景。TrueBlack®還用于猝滅聚碳酸酯濾光片上的背景熒光，所述聚碳酸酯濾光片用作成像細胞術的細胞支持物。
- 產品形式
- 該TrueBlack?脂褐素自體熒光淬滅劑，20 X DMF溶液產品形式待更新請參考產品說明書
- 保存建議
- 室溫
- 其他
- Biotium公司是一家在熒光技術領域獨樹一幟的生物技術公司，公司位于美國加利福尼亞的BAY AREA------中外知名首要的生物科技中心。該公司一直致力于開發和生產用于生物醫學研究的熒光指示劑及其他特殊生化產品。其產品被廣泛應用于細胞生 物學、蛋白質組學、分子生物學、免疫學、微生物學、診斷學、生物化學、神經科學、血液流動檢測及大規模藥物篩選等眾多領域。Biotium公司專利產品眾多，包括已經被廣泛使用的實時PCR定量熒光染料EvaGreen、具有劃時代意義的凝膠核酸染料Gel Red和GelGreen，以及最新推出的Cheetah Taq熱啟動酶和CF系列蛋白質標記染料等。 n目前，Biotium已將其許多專利技術授權給一些主要的國際生物技術公司，包括安捷倫、BD生物科學、Biolegend、BioRad、EMD Millipore、Illumina、PerkinElmer、Qiagen、Roche、Sartorius AG、Sigma-Aldrich、Thermo Fisher Scientific等。
- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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