10-Acetyl-3,7-dihydroxyphenoxazine (N-Acetyl-3,7-dihydroxyphenoxazine or ADHP), which has been marketed under the name Amplex® Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H2O2(reacting in a 1:1 stoichiometry) in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. H2O2 is produced by many enzymatic reactions, and thereforeADHP can be used to detect the activity of many different enzymes.
Detect HRP in immunoassays
Detect trace amounts of hydrogen peroxide (H2O2)
Detect using fluorometer or microplate reader
λEx/λEm(pH 9)= 571/585 nm (end product)
ε= 54,000 (end product)
White to off-white solid soluble in DMSO or DMF
Store desiccated at -20°C and protect from light
C14H11CNO4
MW: 257.24
[119171-73-2]
Amplex Red is a registered trademark of Thermo Fisher Scientific.
產品描述
10-乙?；?3，7-二羥基吩惡嗪（N-乙?；?3，7-二羥基吩惡嗪或ADHP），其已經以名稱Amplex銷售®紅色被認為是過氧化物酶的最佳熒光底物，因為它具有高度特異性和穩定性。底物本身在被H氧化前幾乎是無色無熒光的 2 O 2在辣根過氧化物酶（HRP）的存在下，將所述化合物（以1：1化學計量反應）轉化為高度紅色熒光試鹵靈。H 2 O 2由許多酶促反應產生，因此ADHP可用于檢測許多不同酶的活性。
