ATP Standard 10 nM is diluted in Extractant B/S and is used in hygiene control assays. The ATP Standard is stable in liquid form and certified by the Swedish Standards Institute.nnATP measurements are like most measurements subjects to inhibition and other variables. These parameters could be known or unfamiliar to the user and their significance may vary between measurements.nnThe RLU output from the same amount of ATP will vary with temperatur, pH, ATP reagents, different Luminumeters and very often with the sample matrix.nnCalibration with ATP standard addition (i.e know amount of ATP) accounts for:n- Variations between luminometers, pH and temperaturen- Inhibition or quenching of light from the samplen- Differences in the sensitivity of reagentsnnnAs a result the the usage of ATP standard makes it possible to convert the RLU result of your sample to amounts of ATP (pmol). The ATP Standard is delivered as a stable solution of known concentration. The method is very easy and rapid. Immediately after you take the light reading from your sample, just add the ATP standard to your sample and measure the light emitted. The amount of ATP in your sample can be simply calculated as follows:nnATP smp = 1 x I smp / ( I smp + std – I smp )nnI smp is the light emission from the sample I smp + std is the light emission from the sample plus ATP standard The factor 1 is the amount of ATP Standard added (i.e. 1 pmol).nnInstructions on the use of the ATP Standard accompany each assay kit.
產品描述
ATP standard makes i possible to express ATP measurements in moles rather than RLU.nATP Standard 10 nM is diluted in Extractant B/S and is used in hygiene control assays. The ATP Standard is stable in liquid form and certified by the Swedish Standards Institute.nnDelivery form:nSingle reagentnnNumber of assays:n6,7 mL allows up to approx. 220 assays.nnStorage:nAt +4 °C or lower.nnShelf life:n2 years from date of delivery at +4 °C.
產品形式
保存建議
4 °C
其他
BioThema成立于1987 年，總部位于瑞典斯德哥爾摩南郊，只做一件事：把螢火蟲熒光素酶反應做到極致。38 年來，公司依舊保持“小而?！钡囊幠?，卻憑這一件事成為全球 ATP 與生物冷光分析的隱形冠軍。nBioThema致力于螢火蟲熒光素酶反應的分析應用。這種多功能反應已廣泛用于各種檢測，包括細胞數量定量、細胞增殖、細胞毒性、參與ATP形成或降解反應的所有酶和代謝物分析、報告基因檢測、免疫檢測、快速細胞計數和環境衛生監測。同時BioThema也是全球很多品牌的ATP和熒光素的OEM原料供應商；
