ATP Reagent SL-M is specially developed for the assay of mithocondrial ATP metabolism, monitoring of oxidative phosphorylation with different substrates as described in Wibom, Lundin & Hultman (1990) Scand J Clin Lab Invest 50:143-152. The reagent can be used for monitoring adenosine triphosphate (ATP) over the range 10-12 – 10-6 mol/L. In contrast to our normal ATP Reagent SL 11-501 it does not contain magnesium ions. This makes the reagent useful for situations when the concentration and counterion of the magnesium salt used in the normal reagent is not optimal. The reagent has a decay of ATP and light emission of less than 1% per minute. The stable light allows manual handling and you can use a simpler luminometer. The detection limit is 10-15 mole ATP, making the reagent suitable for assays of mithocondrial ATP production rate down to the detection limit and for other continous monitoring of enzymatic reactions where the magnesium ion concentration has to be adjusted.
產品描述
The detection limit is 10-15 mole ATP, making the reagent suitable for assays of mithocondrial ATP production rate down to the detection limit and for other continous monitoring of enzymatic reactions where the magnesium ion concentration has to be adjusted.nnDelivery form: Lyophilised reagent in a glass vial, sealed under argonnnReconstitution: In 23-101 Diluent C. A further 5 fold dilution in Tris-EDTA buffer or other suitable buffer is recomended, for maximum performance.nnNumber of assays: Up to 250 analyses in a 96 well microplate format or 50 analyses in cuvette format.nnLinear range: From 10-15 mole to 10-12 mole ATP, depending on the linearity of the luminometer.nnStorage: +4°CnnShelf life: 2 years from date of delivery at +4°C. Reconstituted reagent should be used within a week.
產品形式
保存建議
4 °C
其他
BioThema成立于1987 年，總部位于瑞典斯德哥爾摩南郊，只做一件事：把螢火蟲熒光素酶反應做到極致。38 年來，公司依舊保持“小而?！钡囊幠?，卻憑這一件事成為全球 ATP 與生物冷光分析的隱形冠軍。nBioThema致力于螢火蟲熒光素酶反應的分析應用。這種多功能反應已廣泛用于各種檢測，包括細胞數量定量、細胞增殖、細胞毒性、參與ATP形成或降解反應的所有酶和代謝物分析、報告基因檢測、免疫檢測、快速細胞計數和環境衛生監測。同時BioThema也是全球很多品牌的ATP和熒光素的OEM原料供應商；
