- 中文名稱
Arf1 Pulldown 活化檢測試劑盒
- 英文名字
- Arf1 Pulldown Activation Assay Kit
- 供應商
- Cytoskeleton
- 產品貨號
- BK032-S
- 產品報價
- ￥詢價/KIT20assays
- 產品說明書
- 點擊查看
- 購買方式
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- 產品新聞
- 背景資料
- 該Arf1 Pulldown 活化檢測試劑盒產品是Cytoskeleton品牌原裝進口，Cytoskeleton品牌是細胞骨架研究專家，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發與生產。艾美捷作為中國區域的總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立專業技術支持團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- 收到該Arf1 Pulldown 活化檢測試劑盒Arf1 Pulldown Activation Assay Kit產品后，查看廠家提供的產品說明書詳細介紹。
- 產品特點
- The mammalian ADP-ribosylation factor (Arf) subfamily of Ras-related small G-proteins was originally named for the ability to stimulate cholera toxin mediated ADP-ribosylation of the Gsα subunit utilized by many GPCRs (1). The Arf GTPases have been grouped into three classes based on their size and amino acid similarity (2): class I (Arf1 and Arf3), class II (Arf4 and Arf5) and class III (Arf6). Arf1 functions in the anterograde (and retrograde) transport of proteins from the endoplasmic reticulum (ER) through the Golgi apparatus to the plasma membrane by activating lipid modifying enzymes and recruiting proteins needed to promote secretory vesicle development, scission, and transport between secretory compartments (3, 4, and reviewed in 5). Arf1 has also been shown to function in the maintenance of structural integrity of the Golgi and ER (6).
Arf1, like other small G-proteins, cycles between the inactive GDP-bound and the active GTP-bound states. The preferential association of effector proteins with the GTP-bound over the GDP-bound state of Arf1 provides the basis for Arf1’s function in the cell. This highly specific association of effector proteins with Arf1-GTP has been exploited to develop affinity precipitation assays to monitor Arf1 activation (7).
Cytoskeleton’s Arf1 Activation Assay Biochem Kit? utilizes the Arf1 protein binding domain (PBD) of the effector protein GGA3 (Golgi-localized γ-ear containing, Arf-binding protein 3), which has been shown to specifically bind the GTP-bound form of Arf1 (7, 8). We have covalently conjugated purified GGA3-PBD (amino acids 1-316) expressed in E. coli to the colored sepharose beads provided in this kit (Figure 1). Using these beads, the researcher is able to “pull-down” Arf1-GTP and quantify the level of active Arf1 with a subsequent Western blotting step using the Arf1 specific antibody provided in this kit. This assay provides a simple means of analyzing cellular Arf1 activation levels in a variety of systems. A typical Arf1 pull-down assay is shown in Figure 2 below using either GTPgS and GDP loaded MDCK cell extracts or extracts from C2C12 myoblasts that have been induced to differentiate by serum starvation.
- 保存建議
- 廠家推薦常溫運輸，收到產品后建議保存于4°C。
- 其他
- Cytoskeleton公司成立于1993年，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發與生產。公司提供藥物篩選、信號轉導、蛋白質轉錄后修飾（PTM）、細胞骨架研究相關的系列試劑盒和產品，尤其以細胞骨架相關研究見長，既能滿足于樣品較少的科學研究，也可以用于小規模篩選研究和高通量大規模篩選研究。此外，公司還提供微管蛋白，肌動蛋白，小G蛋白，GAPs，GEFs等現有產品的藥物篩選服務。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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