該微管結合蛋白 Spin Down 檢測試劑盒產品是Cytoskeleton品牌原裝進口,Cytoskeleton品牌是細胞骨架研究專家,專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發與生產。艾美捷作為中國區域的總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立專業技術支持團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
收到該微管結合蛋白 Spin Down 檢測試劑盒Microtubule Binding Protein Spin Down Assay Kit產品后,查看廠家提供的產品說明書詳細介紹。
產品特點
This assay allows the identification of proteins that will bind to microtubules (MTs) in vitro. The assay relies on the fact that MTs will pellet when centrifuged at 100,000 x g. Therefore, any protein that is associated with the MTs will pellet with them during centrifugation. A simple SDS-PAGE analysis of the supernatant versus pellet fraction will identify if a protein is able to associate with MTs. The assay description given in this manual is for recombinantly expressed “test” proteins, however, the assay can be adapted for cell lysates or in vitro translation products.
It should be noted that in vivo confirmation of MT association should be obtained in order to confirm that the protein can be classified as a MAP. This association need not occur throughout the whole cell cycle and may even be developmentally regulated, indeed transient association of MAPs with microtubules is the norm rather than the exception.