Insulin-like growth factor I (IGF-I) also known as Somatamedin C is a hormone similar in molecular structure to insulin. Human IGF-I has two isoforms (IGF-IA and IGF-IB) which are differentially expressed by various tissues. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively. Both IGF-I and IGF-II (another ligand of IGF) can signal through the IGF-I receptor (IGFIR), but only IGF-II can bind the IGF-II receptor (IGFIIR/ Mannose-6-phosphate receptor). IGF-I plays an important role in childhood growth and continues to have anabolic effects in adults. Recombinant Human IGF-I(N-Met) produced in E.coli is a polypeptide chain containing 71 amino acids. A fully biologically active molecule, rhIGF-I (N-Met) has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
序列
MGPETLCGA ELVDALQFVC GDRGFYFNKP TGYGSSSRRA PQTGIVDECC FRSCDLRRLE MYCAPLKPAK SA
來源宿主
Escherichia coli.
溶解建議
Reconstituted in ddH2O or PBS at 100 μg/ml.
保存建議
Lyophilized recombinant Human IGF-I(N-Met) remains stable up to 6 months at -80
