UltraScence Femto Plus Western Substrate,作為一種基于發光氨的增強化學發光底物,是敏感的,與辣根過氧化物酶(HRP)偶聯二抗體進行免疫印跡兼容。UltraScence Femto Plus Western底物的設計目的是檢測數量太小的目標蛋白,用典型的ECL底物看不到。UltraScence Femto Plus Western Substrate的卓越靈敏度和長信號持續時間使抗原的低飛圖檢測(中飛波)成為可能。此外,它的長化學發光信號持續時間使數字和基于薄膜的成像不損失任何信號成為可能。建議適當的一抗和二抗稀釋以獲得最佳的信號強度和持續時間。此外,UltraScence Femto Plus WesternSubstrate的高穩定性使其在室溫下可以存儲2年,而不會對性能產生任何影響。
產品描述
The UltraScence Femto Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate,is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugatedsecondary antibodies. The UltraScence Femto Plus Western Substrate is designed for the detection of the targetproteins in amounts that are too small to be seen with typical ECL substrates. The low femtogram detection (midzeptomole) of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and longsignal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imagingpossible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested forattaining optimal signal intensity and duration. Moreover, the high stability of UltraScence Femto Plus WesternSubstrate makes a 2-year- storage at room temperature possible, without any compromise on the performance.