- 中文名稱
清潔帽
- 英文名字
- Clean Cap
- 供應商
- Baseclick
- 產品貨號
- BCT-38-S
- 產品報價
- ￥詢價/1umol
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- 清潔帽(Clean Cap)背景資料,請查看該產品的使用說明書
- 產品描述
- Messenger RNA (mRNA) serves numerous research and clinical purposes. It is produced through the transcription of a DNA template. In vitro transcription (IVT) of mRNA requires efficient capping strategies to generate biologically active and stable mRNA molecules. In mammalian cells, specific molecular structures are added to one end of an mRNA strand to shield it from degradation and ensure proper translation and function. This process is known as capping. Typically, both in vivo and in various lab procedures, the cap is attached after mRNA synthesis, referred to as post-transcriptional or enzymatic capping. This method necessitates additional enzymatic reactions and purification steps, increasing the complexity and duration of the process.TriLink Biotechnologies provides a co-transcriptional capping technology using the CleanCap? molecule, which offers a streamlined, one-pot solution that minimizes the number of steps and results in a cap-1 structure crucial for mRNA function. This, CleanCap?-AG Reagent (BCT-38) is a trinucleotide reagent containing the native cap structure and an A and G at the initial two positions of the mRNA.Conventional capping methods, such as the use of ARCA (anti-reverse cap analogue), sometimes face the problem of capping efficiency and cost. These methods usually result in a cap 0 structure, which can activate pattern recognition receptors and lead to immune responses, reducing the overall efficacy of the mRNA.The CleanCap?-AG molecule binds to the active site of T7 RNA polymerase when it attaches to the DNA template, stabilizing the site. The AG bases of the CleanCap? molecule form base pairs with the DNA template. T7 RNA polymerase can then extend from the CleanCap? molecule by adding new bases, including various modified NTPs, to produce capped RNA products that closely resemble natural mammalian mRNA.CleanCap? reagents advantages: facilitate efficient manufacturing, achieving 95% capping efficiency yielding 3x more product than traditional co-transcriptional methods unlike older techniques reduce production time decrease mRNA immunogenicity enhance stability create the ideal cap-1 structure for superior in vivo performance.PLEASE NOTE: CleanCap? Reagent AG requires an AG initiator.
- 產品形式
- Liquid
- 保存建議
- -20 °C, 避光, 干燥, 無RNA酶
- 其他
- Baseclick于2008成立于2008年德國慕尼黑，BaseClick的核心競爭力源于其專有的銅催化疊氮-炔環加成（CuAAC）點擊化學技術。該技術由2001年諾貝爾化學獎得主Barry Sharpless教授發明，BaseClick擁有在核酸領域應用該技術的全球獨家許可。相比傳統方法，點擊化學具有反應迅速、條件溫和、產率高、選擇性強等顯著優勢，可實現從小分子寡核苷酸到大基因片段的高效、精準功能化修飾。目前的應用領域包括：核酸標記技術、Edu細胞增殖試驗，PCR標記試劑盒，FISH探針，（高度）修飾的寡核苷酸等等，Baseclick跟Thermo Fisher，Roche，Merck，abcam，BD Bioscience等諸多知名品牌建立了合作關系。
- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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