PE-Texas Red is a tandem fluorescent dye composed of PE and Texas Red. Antibodies labeled with PE-Texas Red are compatible with flow cytometers equipped with a 488nm argon laser. PE-Texas Red has a maximum emission wavelength of 615nm, which can be detected in the FL3 channel of a flow cytometer. Antibodies labeled with PE-Texas Red can be used in small flow cytometers with milliwatt-level lasers and large flow sorters equipped with full-power lasers. Activated PE is easily conjugated to antibodies and other proteins without the need for chemical crosslinkers. These highly purified PE conjugates retain their spectral properties after conjugation. Our activated PE is treated with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), which reacts with lysine residues. The maleimide group can react with free sulfhydryl groups of the conjugate partner protein. They are ready-to-use and can be directly conjugated without further pre-processing when mixed with sulfhydryl-containing targets.
產品描述
PE-Texas Red 是由 PE 和 Texas Red 組成的串聯熒光染料。PE-Texas Red 標記的抗體與配備 488nm 氬激光器的流式細胞儀兼容。PE-Texas Red 的最大發射波長為 615nm，可在流式細胞儀的 FL3 通道中檢測到。用 PE-Texas Red 標記的抗體可用于配備毫瓦級激光器的小型流式細胞儀和配備全功率激光器的大型流式細胞儀?；罨?PE 很容易與抗體和其他蛋白質偶聯，無需化學交聯劑。這些高度純化的 PE 偶聯物在偶聯后仍可保留其光譜特性。我們的活化 PE 經過琥珀酰亞胺基 4-（N-馬來酰亞胺甲基）環己烷-1-羧酸鹽 （SMCC） 處理，它與賴氨酸殘基反應。馬來酰亞胺基團可與偶聯伴侶蛋白的游離巰基反應。它們是即用型，與含巰基的靶標混合時，無需進一步預處理即可直接偶聯。
