中文名稱

AgriseraTMB HRP底物（100毫升）

英文名字

AgriseraTMB HRP Substrate(100 ml)

供應商

Agrisera

產品貨號

AS15-TMB-HRP

產品報價

￥已停產

產品說明書

點擊查看

購買方式

銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868。

產品新聞

背景資料

3,3',5,5'-Tetramethylbenzidine, horseradish peroxidase (AS15 TMB-HRP) is a sensitive reagent that is ready to use for the quantitative detection of HRP bound to a solid phase or in free solution. It is designed to detect and quantify specific peptide/protein/hormone in a complex mixture in enzyme linked immunosorbent assay (ELISA). It utilizes the enzymatic reaction triggered by horse raddish peroxidase (HRP) in which a chromogenic, chemiluminiescent or chemifluorescent substrates are oxidized resulting in color change, chemiluminiscence or chemifluorescence respectively. A one-electron oxidation product is formed in the presence of HRP and hydrogen peroxide. This is a cation free radical, it is blue in color with adsorption maximum at 653 nm. The a reaction with HRP, hydrogen peroxide or acidification of the radical with acid yields the diimine terminal oxidation product that adsorbs light at 450 nm. The extinction coefficient of the radical ( E653 nm = 3.9 x 104 mol-1 cm-1 ) and diimine ( E450 nm = 5.9 x 104 mol-1 cm-1 ) provides a very sensitive system for this assay.After incubations with antibodies or HRP labeled reagents, TMB solution is added. As an alternative TMB can also be spiked with a different volume of buffered HRP to create dilution series of this substrate, for example 50 %, 70%. This will change sensitivity of this product and can make it more suitable for certain assays, depending what kinetics are required. If you require to adjust sensitivity of your assay, please check Agrisera TMB/Diluent pack. The oxidation of TMB by HRP produces a blue reaction product, measured at 650 nm. Color formation can be recorded as a function of time or the reaction can be stopped using an equal volume of 0.3 M sulfuric acid after a fixed interval. Increased sensitivity is achieved by converting the blue radical to the yellow diimine by addition of acid. The resulting yellow chromogen is measured immediately at 450 nm.

產品描述

AgriseraTMB HRP底物（100毫升）該產品的詳細產品描述特點可查看對應的英文產品說明書。

產品形式

AgriseraTMB HRP Substrate(100 ml)產品形式可查看廠家提供的產品說明書，更多疑問可咨詢在線客服為您解答。

保存建議

Stable for 3 years at +2-8° C or at room temperature up to six months, and can also be frozen. Storge at room temperature for 1 month results in ca. 2 % activity loss. Protect the substrate from exposure to sunlight.

其他

Agrisera公司提供1000種用于植物和藻類細胞生物學研究的一抗，2000多種二抗。Agrisera公司提供的植物領域的抗體涵蓋擬南芥、萊茵衣藻、藍藻、松柏類、硅藻、大豆、大麥、蒺藜苜蓿、煙草、稻、菜豆、小立碗蘚、楊樹、豌豆、番茄、馬鈴薯、菠菜、小麥、玉米、葡萄等多個物種，此外還有水生生物類（藻類、魚）抗體，細菌、昆蟲、真菌抗體。針對植物蛋白質的保守氨基酸序列，Agirsera還獨家開發了數十種通用抗體（global antibodies），這類抗體幾乎可以與所有的高等植物及藻類產生免疫反應，可為研究人員提供極為方便的選擇。

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

分享到：