PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the number of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference). The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6
應用類型
protein extraction
免疫原
該1 | PEB（4x）|?蛋白質提取緩沖液產品的免疫原查看產品說明書。
來源宿主
該1 | PEB（4x）|?蛋白質提取緩沖液產品的來源宿主查看產品說明書。
反應性
PEB has been tested on a wide range of species and tissues from Higher plants, Mosses, Llichens, Algae, Diatoms, Dinoflagellates, Cyanobacteria. Extracts may be quantified using detergent (LDS) compatible methods, and have been shown to give highly reproducible and quantitative results in subsequent SDS PAGE gel electrophoresis, Western blotting, and Immunoprecipitation (IP). Most of Agrisera commercial antibodies are tested on plant or algal samples extracted with this buffer. An example can be found here.
保存建議
Stable at RT for at least 1 month; short-term storage (6 monthss) at 4
