Ecto-5-prime-nucleotidase （5-prime-ribonucleotide phosphohydrolase） catalyzes the conversion of purine 5-prime mononucleotides to nucleosides， the preferred substrate being AMP at a neutral pH. CD73， which consists of a dimer of 2 identical 70kDa subunits， is bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. CD73 is used as a marker of lymphocyte differentiation. Consequently， a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Additional forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities， requirement for divalent magnesium ion， activation by ATP， and inhibition by inorganic phosphate.
應用類型
ELISA，WB
免疫原
Anti-human CD73 mAb ， is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CD73 amino acids 27-252 purified from E. coli.
