- 中文名稱
IGFBP-3，完整 ELISA
- 英文名字
- IGFBP-3, Intact ELISA
- 供應商
- Ansh Labs
- 產品貨號
- AL-149
- 產品報價
- ￥詢價/96wellmicrotiter
- 產品說明書
- 點擊查看
- 購買方式
- 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- Insulin-like growth factor binding protein-3 (IGFBP-3) is a 264-amino acid peptide (MW 29 kD) produced by the liver as well as other tissues. It is the most abundant of a group of IGFBPs that transport, and control bioavailability and half-life of insulin-like growth factors (IGF), in particular IGF-I, the major mediator of the anabolic- and growth-promoting effects of growth hormone (GH).1 The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II, thus prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Most of the IGFBP-3 in plasma is present as the high molecular weight ternary complex, however, small amounts of free IGFBP-3 are also found.2,3
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nIGFBP-3 also exhibits intrinsic growth-regulating effects that are not yet fully understood, but have evoked interest with regards to a possible role of IGFBP-3 as a prognostic tumor marker. Low IGFBP-3 and IGF-I levels are observed in GH deficiency or GH resistance,4 also epidemiologic studies suggest that low IGFBP-3 is associated with greater risk of aggressive, metastatic prostate cancers.5 The elevated serum IGFBP-3 and IGF-I levels indicate a sustained overproduction of GH, or excessive rhGH therapy. Both conditions are associated with generalized organomegaly,6 hypertension,7 diabetes,8 cardiomyopathy,9 osteoarthritis,10 and diminished longevity.
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nThe Ansh Labs Intact IGFBP-3 Assay uses an acidification and neutralization method to dissociate Intact IGFBP-3 from all the binding subunits. Intact IGFBP-3 levels are quantified in the extracted samples using a highly sensitive and specific Intact IGFBP-3 ELISA. Total IGFBP-3 levels can be measured using Total IGFBP-3 ELISA (AL-120).
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nThe concentration of bioactive IGF-I, total IGFBP-3 and intact IGFBP-3 in biological fluid can be measured accurately using immunoassay methods (Bioactive IGF-1 ELISA; AL-122, IGF-I inhibition mAb capture and IGF-1 C-terminal mAb) detection; Total IGFBP-3 ELISA; AL-120, C-terminal IGFBP-3 capture and detection mAb; and Intact IGFBP-3 ELISA; AL-149, C-terminal mAb capture and N-terminal mAb detection, respectively). The ratio of total to Intact IGFBP-3 concentration measured in individual subject over time will help normalizes the IGFBP-3 variability between subjects. The immunoassay methods designed for the measurement of bioactive IGF-I, total and Intact IGFBP-3 in patient samples could be of practical value for the diagnosis or prediction of various pathologies including growth abnormalities and cancer.
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nReferences:
n1. Hwa V, Oh Y, Rosenfeld RG. The insulin-like growth factor-binding protein (IGFBP) superfamily, Endocr Rev. 1999 Dec;20 (6):761-87.
n2. Baxter RC, Martin J : Radioimmunassay of growth hormone-dependent insulin-like growth factor binding protein in human plasma. J Clin Invest 1986, 78:1504-1512
n3. Blum WF, Ranke MB, Kietzmann K, Gauggel E, Zeissel HJ, Bierich JR. A specific radioimmunoassay for the growth hormone (GH)-dependent somatomedin-binding protein: its use for diagnosis of GH deficiency. J Clin Endocrinol Metab, 1990, 70:1292-1298
n4. Wetterau L, Cohen P: Role of insulin-like growth factor monitoring in optimizing growth hormone therapy. J Ped Endocrinol Metab 2000;13:1371-1376
n5. Cancer Res August 1, 2011 71;5154 IGFBP-3 Is a Metastasis Suppression Gene in Prostate Cancer
n6. Parama C, Fluiters E, de la Fuente J, et al: Monitoring of treatment success in patients with acromegaly: the value of serum insulin-like growth factor binding protein-3 and serum leptin measurements in comparison to plasma insulin-like growth factor 1 determination. Metabolism 2001; 50: 1117-1121.
n7. Watanabe T1, Itokawa M, Nakagawa Y, Iguchi T, Katagiri T. Increased levels of insulin-like growth factor binding protein-3 in hypertensive patients with carotid atherosclerosis. Am. J. Hypertens. 2003 Sep; 16 (9 Pt 1):754-60.
n8. Kim MS, Lee DY. Serum insulin-like growth factor-binding protein-3 level correlated with glycemic control and lipid profiles in children and adolescents with type 1 diabetes. J Pediatr Endocrinol Metab. 2014 Sep 20;27(9-10):857-61. doi: 10.1515/jpem-2013-0358.
n9. Granata R, Broglio F, Migliorino D, Cutrupi S, Baldanzi G, Sireno M, Fubini A, Grazian A, Ghigo E, Pucci A. Neonatal and adult human heart tissues from normal subjects and patients with ischemic, dilated or hypertrophic cardiomyopathy express insulin-like growth factor binding protein-3 (IGFBP-3). J Endocrinol Invest. 2000 Dec;23(11):724-6.
n10. Lee HS, Woo SJ, Koh HW, Ka SO, Zhou L, Jang KY, Lim HS, Kim HO, Lee SI, Park BH. Regulation of apoptosis and inflammatory responses by insulin-like growth factor binding protein 3 in fibroblast-like synoviocytes and experimental animal models of rheumatoid arthritis. Arthritis Rheumatol. 2014 Apr;66(4):863-73. doi: 10.1002/art.38303.
n11. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
n12. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 - Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
n13. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.
- 產品描述
- The Intact IGFBP-3 enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of intact IGFBP-3 in human serum and other biological fluids.
- 產品特點
- 保存建議
- Store at 2 to 8°C until expiration date.
- 其他
- Ansh labs是開發和生產免疫檢測試劑盒和定制生物技術檢測產領創者，總部位于美國休斯敦，公司產品遍及全球80多個國家。公司于2011年成立，核心團隊來自于Diagnostic system laboratory，專注于女性健康和激素檢測領域近40年，擁有全球領先的核心技術， 以及多年經驗生物技術研發經驗的科學家團隊，產品覆蓋生殖健康，高危妊娠，腫瘤，糖尿病等多個疾病領域，圍繞最新的免疫分析技術，多種蛋白和單克隆抗體，如AMH，BMP-15, Follistatin, GDF-9, IGF-I, IGF-II, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, Inhibin Alpha, Inhibin ?A, Inhibin ?B, MBP, PAPP-A, PAPP-A2 and Vitamin D.等幾十余種試劑產品，也同時涵蓋小鼠，大鼠，狗，羊，牛等靈長類多個物種的獨特的物種特異性的試劑。
Ansh labs 坐落于美國德克薩斯州，是目前世界上唯一能提供TGF-β超家族所有成員的檢測公司，也是最早專注激素類產品研究和新型免疫檢測技術的先驅者。優勢產品涉及TGF-β super family、AMH/抑制素B(human/animal)、full panel IGFs、以及最完整的胰高血糖素系列。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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