Recognizes a protein of 130-140kDa，identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny，CD22 is first expressed in the cytoplasm of pro-B and pre-B cells，and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells，CD22 is found highly expressed in follicular mantle and marginal zone B-cells，and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
應用類型
FC, IF, IHC, WB, ELISA
免疫原
Recombinant human BL-CAM/CD22 protein (aa52-178) (exact sequence is proprietary)
