Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).|Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.|CXCL9 is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). It is secreted by various cell types including immune cells (T lymphocytes, NK cells, macrophages) and non-immune cells (fibroblasts, keratinocytes, endothelial cells). The main factor of production is the interferon-gamma [2] in Th1-type immune responses. CXCR3 has been identified as the receptor of CXCL9. The main function of CXCL9 is the recruitment of immune cells on the inflammation site.
