- 中文名稱
宿主細胞蛋白（大腸桿菌）ELISA試劑盒
- 英文名字
- Host Cell Proteins (E.coli) ELISA kit
- 供應商
- Bertin Bioreagent
- 產品貨號
- A05034
- 產品報價
- ￥詢價/96wells
- 產品說明書
- 點擊查看
- 購買方式
- 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).|Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.|Impurity assessment is a key step during the drug development production of recombinant proteins, including therapeutic proteins. Specific impurities coming from the cells mediating the protein expression, known as Host Cell Proteins (HCP) are generated and need to be removed. This kit is intended for use in assessing relative quantities of E. coli HCP in manufactured or research bioproducts.|Polyclonal antibodies used in this kit have been generated against several strains of E.coli and specifically selected for their recognition of a large spectrum of E. coli proteins. Thus, this kit can be considered as generic and allows a relative-quantitative determination of E. coli HCP in many types of samples, such as samples issued from the purification process (HCP clearance), process control, quality control, or product release.|Using this kit, HCP concentration is measured in ng/ml (HCP equivalent is extrapolated from a standard curve). Conventionally, the HCP content in a product will finally be expressed in ng/mg, where ng represents HCP mass and mg represents the product mass. Note that, contrary to the concentration measurement of the product, the HCP signal is only reflective of antibody binding and does not strictly reflect the mass of HCP.|This kit has been successfully validated for recovery and precision using reconstituted HCP samples and tested against different final products. Given the diversity of final products, all potential matrix effects cannot be known and it is recommended that you test the suitability of the kit for use with your own HCP samples in your laboratory.|This kit should be used as one part of your complete HCP analysis.
- 產品描述
- 酶免疫測定（EIA）是一種檢測和定量樣品中抗原（蛋白質、激素等）或抗體的技術。它依賴于抗體結合特定抗原的能力。用酶標記抗體或抗原，所述酶的底物是轉化成可測量產物（顏色或熒光）的色原或熒光團。|酶聯免疫吸附測定（ELISA）是使用固相（例如：微量滴定板）上包被有固定分子的抗原以進行檢測。隨著時間的推移，科學家們已經將術語ELISA擴展到使用抗體包被固相的EIA。這就解釋了為什么我們使用包被抗體的EIA試劑盒也被稱為ELISA試劑盒。|雜質評估是重組蛋白（包括治療性蛋白）的藥物開發生產過程中的關鍵步驟。來自介導蛋白質表達的細胞的特定雜質（稱為宿主細胞蛋白質（HCP））被產生并且需要被去除。該試劑盒預期用于評估大腸桿菌的相對量。生產或研究生物制品中的大腸桿菌HCP。|該試劑盒中使用的多克隆抗體已經針對幾種大腸桿菌菌株產生，并且針對它們識別大范圍的大腸桿菌而被特異性地選擇。coli蛋白。因此，該試劑盒可被認為是通用的，并允許相對定量測定E。在許多類型的樣品中，例如從純化過程（HCP清除）、過程控制、質量控制或產品放行中發出的樣品，可以檢測到大腸桿菌HCP。|使用該試劑盒，以ng/ml測量HCP濃度（HCP當量從標準曲線外推）。通常，產品中的HCP含量最終以ng/mg表示，其中ng表示HCP質量，mg表示產品質量。請注意，與產品的濃度測量相反，HCP信號僅反映抗體結合，并不嚴格反映HCP的質量。|已使用復溶HCP樣品成功驗證了該試劑盒的回收率和精密度，并針對不同的最終產品進行了檢測。鑒于最終產品的多樣性，無法了解所有潛在的基質效應，建議您在實驗室檢測試劑盒與您自己的HCP樣本一起使用的適用性。|該試劑盒應作為完整HCP分析的一部分使用。
- 產品特點
- 該宿主細胞蛋白（大腸桿菌）ELISA試劑盒,Host Cell Proteins (E.coli) ELISA kit產品產品特點詳情查看產品說明書。
- 保存建議
- Store at +4degrees; shelf life 6 months maximum after production
- 其他
- 法國Bertin Technologies公司是一家大型儀器設備及試劑供應商，擁有50多年的歷史，產品涉及衛生、環境、安全甚至軍工等諸多領域。Bertin Bioreagent是Bertin Technologies集團的生物試劑部門，主要是致力于研發與炎癥，氧化損傷，內分泌學，糖尿病，肥胖癥，高血壓，精神藥理學，癌癥，哮喘，朊病毒疾病，藥代動力學和代謝等相關的化學試劑 ，蛋白，底物，抗體及檢測試劑盒等產品。 Bertin Instruments是Bertin Technologies集團的儀器部門，其中旗艦產品Precellys系列多功能生物樣品勻漿器是全世界各個實驗室的明星產品，截至目前累計有15000+的引文！
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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