- 中文名稱
TrueBlot? Immunoprecipitation and Western Blot Kit for GFP Epitope Tag - 88-8887-215
- 英文名字
- TrueBlot? Immunoprecipitation and Western Blot Kit for GFP Epitope Tag - 88-8887-215
- 供應商
- Rockland
- 產品貨號
- 88-8887-215
- 產品報價
- ￥詢價/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- TrueBlot? Immunoprecipitation and Western Blot Kit for GFP Epitope Tag allows for the immunoprecipitation and detection of GFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-GFP. Detection of the membrane bound antibody-antigen complex is achieved by the addition of Mouse IgG TrueBlot? ULTRA HRP. The TrueBlot? Immunoprecipitation and Western Blot Kit for GFP Epitope Tag contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and western blot containing a GFP Epitope Tag using TrueBlot secondary antibody immunoblotting reagents in conjunction with Rockland's Anti-GFP (MOUSE) Monoclonal Antibody. Mouse IgG TrueBlot? ULTRA is the unique horseradish peroxidase conjugated Anti-Mouse IgG monoclonal secondary antibody which enables detection of immunoblotted target protein bands, without hindrance from interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your conventional HRP Anti-Mouse IgG secondary antibody. It is easy to generate publication-quality IP/WB data with Mouse IgG TrueBlot? ULTRA.
- 產品描述
- Mouse IgG TrueBlot? ULTRA is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). Mouse IgG TrueBlot? ULTRA is an HRP-conjugated monoclonal secondary antibody reacting with mouse IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. TrueBlot? IP and WB Kit for GFP Epitope Components: 1. Mouse IgG TrueBlot ULTRA: 50 μL 18-8817-31; 2. TrueBlot Enhancer Solution: 25 mL; 3. TrueBlot Blocker: 10 g; 4. TrueBlot Assay Buffer: 30 mL 20X; 5. TrueBlot Substrate A: 12.5 mL; 6. TrueBlot Substrate B: 12.5 mL; 7. Anti-Mouse Ig IP Beads: 2.5 mL Binds 0.4 mg Ig/mL beads; 8. Anti-GFP (MOUSE) Monoclonal Antibody: 100 μg; 9. Western Blot Incubation Tray. Special Notes: Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer.
- 產品特點
- 該TrueBlot? Immunoprecipitation and Western Blot Kit for GFP Epitope Tag - 88-8887-215的詳細信息查看Rockland提供的產品說明書。
- 保存建議
- Store Kit at 2-8 °C, except Mouse TrueBlot? ULTRA and Anti-GFP (MOUSE) Monoclonal Antibody, which should be stored at -20 °C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.
- 其他
- Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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