- 中文名稱
Mouse TrueBlot? Set (with IP Agarose beads) - 88-7788-31
- 英文名字
- Mouse TrueBlot? Set (with IP Agarose beads) - 88-7788-31
- 供應商
- Rockland
- 產品貨號
- 88-7788-31
- 產品報價
- ￥詢價/1Set
- 產品說明書
- 點擊查看
- 購買方式
- 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- Mouse IgG TrueBlot? is a unique horseradish peroxidase conjugated Anti-Mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot? enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Mouse IgG TrueBlot?, simply substitute the conventional HRP Anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot? and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot? is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot? with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Mouse IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.
- 產品描述
- Mouse IgG TrueBlot? ULTRA is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. Mouse TrueBlot Set Components: 1. Mouse IgG TrueBlot? HRP-conjugated monoclonal secondary antibody reacting with mouse IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments. 2. Anti-Mouse Ig IP Beads: 2.5 mL. Binds 0.4 mg Ig/mL beads. 3. Western blot incubation tray. Special Notes: Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution factor for your desired application. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.
- 產品特點
- 該Mouse TrueBlot? Set (with IP Agarose beads) - 88-7788-31的詳細信息查看Rockland提供的產品說明書。
- 保存建議
- Store TrueBlot? Anti-Mouse Ig IP beads (00-8811-25) at 2-8 °C and Mouse TrueBlot? (18-8817-31) at -20 °C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.
- 其他
- Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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