c-Myc-，N-Myc- and L-Myc-encoded proteins function in cell proliferation，differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half-lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung，breast and colon carcinomas，while the N-Myc gene has been found amplified in neuroblastoma. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus，including the leucine zipper，the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif （bHLH-Zip） protein designated Max，specifically associates with c-Myc，N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins，Mad and Mxi1，and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.
應用類型
Western blot: ~1:1000， FC: 1:10 – 1:50.
免疫原
This MYC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from human MYC.
