A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine （His） residues，followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags，this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
應用類型
ELISA， WB
免疫原
6X-HIS-KLH
來源宿主
小鼠
反應性
經檢測驗證，可與所有種屬來源的His-Tag抗體（克隆號11E12）蛋白發生免疫反應.
保存建議
提供的His-Tag抗體（克隆號11E12）產品為無色溶液形式，含有100 ul抗體.該產品濃度為1 mg/ml，溶解于0.15 M PBS緩沖液中.請在收到該His-Tag抗體（克隆號11E12）產品后，將其置于-20 °C冷凍保存，建議分裝使用以避免反復凍融.
