UHRF1，also named as ICBP90，NP95 and RNF106，is a putative E3?ubiquitin protein?ligase. It may?participate in methylation dependent transcriptional?regulation. UHRF1 binds to inverted 5’-CCAAT-3’?box 2 in?the TOP2A?promoter，and activates TOP2A?expression. It is important for G1/S?transition and may be involved in?DNA?repair and chromosomal stability. UHRF1’s ability to repress its direct target gene expression is dependent on PHD （UHRF1） binding to unmodified H3R2.
應用類型
Western blot: 1:500 – 1:2000， IHC: 1:50 – 1:200， IF: 1:20 – 1:50， IP: 1:20 – 1:50， CHIP: 1:20 – 1:50. However， the optimal conditions should be determined individually.
