Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes （UBEs） and deubiquitinating enzymes （DUBs）. DUBs are categorized into 5 subfamilies: USP，UCH，OTU，MJD，and JAMM. UCHL1，UCHL3，UCHL5/UCH37，and BRCA-1-associated protein-1 （BAP1） belong to the UCH family of DUBs，which all possess a conserved catalytic domain （UCH domain） of about 230 amino acids. UCHL5 and BAP1 have unique extended C-terminal tails. UCHL5 is the only ubiquitin carboxy-terminal hydrolase （UCH）-family protease that is associated with mammalian proteasomes. It is a protease that specifically cleaves ’Lys-48’-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex； however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. It is responsible for the ubiquitin isopeptidase activity in the PA700 （19S） proteasome regulatory complex.
應用類型
Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 ug/mL
