Glutathione S-transferase (GST) is a widely used protein fusion partner,since it provides an easily detectable Tag and also a simple purification process with little effect on the biological function of the protein of interest.
應用類型
Western blotting (0.5-4 ug/ml) and Immunohistochemistry (10-20 ug/ml, frozen & paraffin). Recombinant GST-GSK-3 fusion protein (Cat.#: 7003-100) can be used as a positive control. However, the optimal conditions should be determined individually.
免疫原
GST fusion protein
來源宿主
兔
反應性
經檢測驗證,可與所有種屬來源的GST-Tag蛋白發生免疫反應.
保存建議
提供的GST-Tag抗體產品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)緩沖液中.請將該GST-Tag抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.