Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation，hematopoiesis，stem cell maintenance，gametogenesis，mast cell development，migration and function，and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1，the regulatory subunit of phosphatidylinositol 3-kinase，and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS，RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1，STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1，leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines，probably interleukins.Source：Recombinant protein corresponding to aa26-189 from human KITLG，fused to GST-Tag at N-terminal，expressed in E. coli.Molecular Weight： ~45.5kDAA Sequence：EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVAStorage and Stability： May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
