Intended Use：This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5000-50,000 cells/well. This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.Sample Type：Cell culture： Adherent cells and non-adherent cellsRange：5000-50,000 cells/wellSpecificity：MammalianTest Principle：This Neutral red cell cytotoxicity BioAssay is one of the common methods used to detect cell viability or drug cytotoxicity. The principle of this assay is based on the detection of viable cells via the uptake of the dye neutral red. Neutral red is a eurhodin dye that stains lysosomes in viable cells. Viable cells can take up neutral red via active transport and incorporate the dye into their lysosomes but non-viable cells cannot not take up this chromophore. Consequently，after washing，viable cells can release the incorporated dye in under acidified-extracted conditions. The amount of released dye can be used to determine the total number of viable cells or drug cytotoxicity. The neutral red uptake assay provides a quantitative measurement of the number of viable cells and can be measured at OD 540nm. The Neutral Red Cell Cytotoxicity BioAssay Kit is simple，accurate，and reproducible. It also includes Doxorubicin，as a positive control. Kit Components：100X Neutral Red Staining Solution，1x2ml10X Washing Solution，1x70ml2X Solubilization Solution，1x75ml20mM Doxorubicin，1x100ulStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對Neutral Red Cell Cytotoxicity BioAssay Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
保存建議
建議收到Neutral Red Cell Cytotoxicity BioAssay Kit產品后將其置于-20°C保存。
