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細胞分析試劑盒
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中文名稱

IL-6 BioAssay ELISA Kit (Human) (Interleukin-6,IL-6,B-cell stimulatory factor 2,CTL differentiation factor,CDF,Hybridoma growth factor,Interferon beta-2,IFN-beta-2,IL6,IFNB2)

英文名字
IL-6 BioAssay ELISA Kit(Human)(Interleukin-6,IL-6,B-cell stimulatory factor 2,CTL differentiation factor,CDF,Hybridoma growth factor,Interferon beta-2,IFN-beta-2,IL6,IFNB2)
供應商
USBiological
產品貨號
360524
產品報價
¥1/96Tests
產品說明書
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購買方式
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業??梢詾槟峁┘皶r的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Interleukin 6 (IL-6),also known as interferon-beta 2,26kD protein and B cell stimulatory factor-2 (BSF-2),is a pleiotropic alpha-helical cytokine that plays important roles in acute phase reactions,inflammation,hematopoiesis,bone metabolism,and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22kD - 28kD phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183aa in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions,some of which exhibit antagonistic properties.Sample Type:Cell culture supernatants,serum,plasma,and tissueIntended Use:The Human IL-6 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-6 concentrations in cell culture supernatants,serum,plasma,and tissue.Sensitivity:1pg/mlRange:3.9-250pg/mlSpecificity:Recognizes human IL-6. There was no detectable cross reactivity with any of the tested proteins following IL-1,2,3,4,5,8,10,12,TNF,VEGF,TGF,IFN,and sICAM.Sample Volume:100ul/wellTest Principle:This assay employs the Sandwich immunoassay technique. An anti-h IL-6 monoclonal coating antibody is adsorbed onto microwells. IL-6 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-6 antibody is added and binds to IL-6 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-6 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-6 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-6 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-6 standard dilutions and IL-6 sample concentration determined.Kit Components:Aluminium pouches with a Microwell Plate: 8x12Standard: 2xVialBiotinylated antibody: 2xVialStreptavidin-HRP: 2xVialAssay Buffer (25ml/Vial): 2xVialWash Buffer Concentrate 20x (30ml/Vial): 1xVialTMB Substrate Solution: 1x12mlStop Solution (12ml/Vial): 1xVialPlate Covers- Adhesive strips: 3xstripsInstruction: 1xStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product,centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對IL-6 BioAssay ELISA Kit (Human) (Interleukin-6,IL-6,B-cell stimulatory factor 2,CTL differentiation factor,CDF,Hybridoma growth factor,Interferon beta-2,IFN-beta-2,IL6,IFNB2)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到IL-6 BioAssay ELISA Kit (Human) (Interleukin-6,IL-6,B-cell stimulatory factor 2,CTL differentiation factor,CDF,Hybridoma growth factor,Interferon beta-2,IFN-beta-2,IL6,IFNB2)產品后將其置于4°C至-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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