- 中文名稱
Hepc (Hepcidin) BioAssay ELISA Kit (Rat)
- 英文名字
- Hepc (Hepcidin) BioAssay ELISA Kit (Rat)
- 供應商
- USBiological
- 產品貨號
- 356254
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Hepc (Hepcidin) BioAssay ELISA Kit is for the quantitative detection of Hepc in rat serum, plasma, tissue homogenates and other biological fluids.Range:78.125-5000pg/mlSensitivity:<46.875pg/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Assay Principle:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Hepc antibody was pre-coated onto 96-well plates. And the biotin conjugated Hepc antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrate was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Hepc amount of sample captured on plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Hepc can be calculated.Kit Components:*35654A: Microtiter Strips, 1x48 wells or 1x96 wells *35654B: Standard, 1x1vial or 2x1vial35654C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml35654D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul35654E: Antibody Dilution Buffer, 1x5ml or 1x10ml35654F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul35654G: SABC Dilution Buffer, 1x5ml or 1x10ml35654H: TMB Substrate, 1x5ml or 1x10ml35654J: Stop Solution, 1x5ml or 1x10ml35654K: Wash Buffer, 25X, 1x15ml or 1x30mlStorage and Stability:Store unopened *35654A at 4oC. Once opened, store at -20oC. Store *35654B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well for 90 minutes at 37°C3. add 100ul Biotin antibody working solution to each well for 60 minutes at 37°C4. Aspirate and wash 3 times5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculation of results
- 產品特點
- Hepc (Hepcidin) BioAssay ELISA Kit is for the quantitative detection of Hepc in rat serum, plasma, tissue homogenates and other biological fluids.Range:78.125-5000pg/mlSensitivity:<46.875pg/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Assay Principle:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Hepc antibody was pre-coated onto 96-well plates. And the biotin conjugated Hepc antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrate was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Hepc amount of sample captured on plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Hepc can be calculated.Kit Components:*35654A: Microtiter Strips, 1x48 wells or 1x96 wells *35654B: Standard, 1x1vial or 2x1vial35654C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml35654D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul35654E: Antibody Dilution Buffer, 1x5ml or 1x10ml35654F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul35654G: SABC Dilution Buffer, 1x5ml or 1x10ml35654H: TMB Substrate, 1x5ml or 1x10ml35654J: Stop Solution, 1x5ml or 1x10ml35654K: Wash Buffer, 25X, 1x15ml or 1x30mlStorage and Stability:Store unopened *35654A at 4oC. Once opened, store at -20oC. Store *35654B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well for 90 minutes at 37°C3. add 100ul Biotin antibody working solution to each well for 60 minutes at 37°C4. Aspirate and wash 3 times5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculation of results
- 保存建議
- 建議收到Hepc (Hepcidin) BioAssay ELISA Kit (Rat)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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