The matrix metalloproteinases （MMP） are a family of peptidase enzymes responsible for the degradation of extracellular matrix components，including collagen，gelatin，fibronectin，laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester，lipopolysaccharide （LPS） or staphylococcal enterotoxin B （SEB）. MMP catalysis requires both calcium and zinc. MMP-13 （also designated collagenase-3） is produced by breast carcinomas and degrades collagen type I，II，III.
應用類型
Western blotting （0.5-4 ug/ml）， immunoprecipitation and Immunohistochemistry （works with paraffin imbedded sections）. However， the optimal concentrations should be determined individually. The antibody recognizes the proenzyme （~60 kDa） and the cleaved fragments （34 kDa and 48 kDa） of MMP-13. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 454 of rat MMP-13
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、馬、牛種屬來源的MMP-13蛋白 發生免疫反應.
保存建議
提供的MMP-13抗體 產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該MMP-13抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
